缓冲液配置一般选择pKa/－1=pH,Tris的pKa是8.2,所以不太适宜配6.8的，可选择NaH2PO4－Na2HPO4体系（pKa=7.2）pH=7.2log([conjugatebase]/[acid])100mL的0.1MSodiumPhosphateBuffer从以下溶液配置0.5MNa2HPO4 和0.5MNaH2PO4则配方为Na2HPO4 mlNaH2PO4 pH16.04368 3.956322 5.815.7167 4.2833 5.915.3705 4.629504 6.015.0052 4.994798 6.114.62117 5.378828 6.214.21899 5.78101 6.313.79949 6.20051 6.413.36376 6.636245 6.512.91313 7.086874 6.612.44919 7.550813 6.711.97375 8.026247 6.811.48885 8.51115 6.910.99668 9.00332 7.0配好后记得调节pH磷酸缓冲液（phosphatebuffer）按照下
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关于同志近三年现实表现材料材料类招标技术评分表图表与交易pdf视力表打印pdf用图表说话 pdf
所给定的体积，混合1mol/L的磷酸二氢钠（单碱）和1mol/L磷酸氢二钠（双碱）贮液，获得所需pH的磷酸缓冲液。配制1mol/L的磷酸二氢钠（NaH2PO4·H2O）贮液：溶解138g于足量水中，使终体积为1L;1mol/L磷酸氢二钠（Na2HPO4）贮液:溶解142g于足量水中使终体积为1L。1mol/L磷酸二氢钠（ml）1mol/L磷酸氢二钠（ml）最终pH值8778508157757356856255655104503903302801231501852252653153754354905506106707206.06.16.26.36.46.56.66.76.86.97.07.17.27．磷酸盐缓冲液（1）磷酸氢二钠–磷酸二氢钠缓冲液（0.2）pH0.2 mol/LNa2HPO4(毫升)0.2 mol/LNaH2PO4(毫升)pH0.2 mol/LNa2HPO4(毫升)0.3 mol/LNaH2PO4(毫升)5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.556.551.045.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3Na2HPO4·2H2O分子量=178.05，0.2mol/L溶液为85.61克/升。Na2HPO4·12H2O分子量=358.22，0.2mol/L溶液为71.64克/升。Na2HPO4·2H2O分子量=156.03，0.2mol/L溶液为31.21克/升。Na2HPO4/Na2HPO4·12H2O=
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