HPLC法测定复方铝酸铋片中甘草酸含量

HPLC法测定复方铝酸铋片中甘草酸含量 HPLC???????;???????????y?x?????d?? ?%????,???-?? 3 ,??????,?????H,?????? (???????H??????????,?????????9??150001) ??E???:????:?????;???????????y?x???????d??????????EP????:???hHPLC??,C18???= ??,?h?6?1?? ???????;???????????y?x???????d??EP????:?h???L???L???????t????????,??????????? ????E???2?? ??EP????:?r??????????????????EH????,?P?h???;???????????y?x??????????EP ???V??:?;??????????;?x????;HPLC ?y???(????:R285.5E?E?E????V?1???H:AE?E?E??????6??:1002-2392(2009)04-0056- 02 ???????mE?E????????m5 ????????E)?y??????E:????(????)?6???????1?????9????EP ???,?????%????(),??,???????5,????????????????????EP 3,???????5,zx@11 2005?? ??????,????????EH???h???????R?x?????????????.?? ?;??????,?h???3?????????G?4????????,?????h???? ?t????EP???y?x?????????h?C???y?????t????,?x?? ???????y???x???????0????EH????EH????EH?????????( ???????7???????????h,?8???????r?????M???r???y ?x???????????d??????EP?r??E)?y??????E:2010???? ???1???????????=?A??????,?r???????????h???1?? ??????,?????r???y?x??????????????,???????????? ???h??,???L???????V,?????????x???????1?1?.?(?? ???????????=?d??????????EP 1E??????R???? ?????c1100?????????=??EP?+??(GR),?????? (AR),??????(AR)EP?x????????????(?y???????m?? ??????????????:110731-200511);?;??????????(?? ???????P?V?,??,7?M?m??????,20???n)EP 2E????=???? ???=??:DiamonsilC18???=??(250mm??416mm, 5L?m);??????:?+??-012molΠL??????????-?????? (67s?33s?1);???m:110mLΠmin;????????:250nm;???? ??:10L?LEP 3E??????????? 311E?????????????????E??????????x???????????? 10mg,??50mL?????y,????????45mL,?????C?(???? ??,????,????,??????????????????,????,????(?? 1mL?d?x??????012mg,???5?x????011959mgEP) 312E?????????????????E????;????????10??,???? ????,?9??,????????012g,??25mL?????y,???????? 20mL,?????C?(30min,????,??????????????????,?? ??,????,????????,????EP ??1E?A1B1? 65?? ?yE?E???E?E???E?E???E?E??? ActaChineseMedicineandPharmacology E?E?E?E?E?E?E?E?E? 2009???r37???r4?m Vol137,No14,2009 :2009-04-20:2009-0-17 :2010 :1977- :E-mail:s1981sinacomcn 4E??????????? 411E??3???t????E??????C???(???x???????6?????? ?????????????5,?0???.20???????????????1????, ???0312??????????10???????????5???????????? ??????????EP?h?????????????=???y,?M???R?x???? ?????????????????=???????C???????=??,???R?r?? ???3???t????EP 412E????t?????L??E??????????x????????????2113, 8153,12177,17104,20115,26164mg,?(?d??50mL???? ???y,?0311????????EP???????????=??,?????????M ???1EH?????????????1???????t????,?????????::A =713E+6C-272917,r=019987EP???????R:?r???h 010426??015328mg??mL -1 ?L???????t????EP 413E????;?t????E????8????????????10L?L,???;?? ??6?n,RSD=113%EP 414E??S???t????E??????????????0?)??????????,?? ????1?2??????,??????????EP5?2?????????????? ??RSD=014%,?S???t????EP 415E???????????E??????????d??????????(???????? ????????,????20061099)6??,?(?d?????x???????? ????????(0120132mg??mL -1 )10mL?R??????10mL,?0 ?)??????????????EP????????????1EP ???????R:?r????????????????,???m?????????? ??EP 5E????????? ???;?8?m????????20??????,?)??????????,?? ??????????2EP ??1E??????????? ??????(mg)?????d??(mg)????????????(mg)??????(mg)??????%??????????%RSD 011120119332101323193299129 011113119202101323192199139 011112 011092 11919 11884 210132 210132 31908 31910 98180 100164 991931130% 0110721185021013231909102127 011108119122101323190999120 E?E?32012mgΠ2 E????????????? ?????????d??(mgΠ)?????????d??(mgΠ)?????????d??(mgΠ) 107100121118071015221215200610992012 2071101211290606012017162007052872212 3071104211110080226021417172007052112114 420060901111211080226011415180802031412 52008011016101205041901810190802021411 60610022312130706022110200802011413 70706052116140703022118????1812 6E????? 611E??r???C???y???????d?x????????013g,E)?y???? ??E:2005???????????????x???????1???y????:?d?x ?????0?????????;?A??810%,?????R???????y?d?x ????????2116mgEP?????????E??20???????????? ??,?????d?x?????????h810mg??2312mg????,?????? 1812mg,???d???tEP?????y???????????:??20%,?? ?5?)?????????9?????????t?G???????????d??????, ?????????????r???????d?x???????????x????( H62016)??,?;???A??1610mgEP 612E??=???r?????h???1???y?;?????x?????????d??, ?????A?????6?h?n?????????;?????E?0????????,?o ?.?????h???0?=????????,??20?????????????????: ??35%???????;?c?5??????????,?A???????????0?? ???????r???y?x?????????d??,?????c?5?h???????0 ???????????@,???N???m???H?G???P?????????????? ??????????EP ??75?? 2009???r37???r4?m Vol137,No14,2009 E?E?E?E?E?E?E?E?E? ?yE?E???E?E???E?E???E?E??? ActaChineseMedicineandPharmacology :C42