结核分枝杆菌MPT64
作者：师长宏，安家泽，唐小凤，王晓武，张海，柏银兰，徐志凯 【关键词】 结核分枝杆菌 Immune responses and protective efficacy induced in mice by Mycobacterium tuberculosis MPT64ESAT6 fusion protein 【Abstract】 AIM: To evaluate humoral and cellmediated immune responses by MPT64 and ESAT6 fusion proteins and to test protective efficacy against Mycobacterium tuberculosis (MTB) challenge. METHODS: BALB/c mice were immunized 3 times at 2week interval subcutaneously on the back with fusion protein MPT64ESAT6. In the spleen lymphocytes of immunized mice stimulation index (SI) was measured by MTT method and the level of secreted IFNγ was detected by ELISA. Some of vaccinated BALB/c mice by fusion proteins were intravenously infected with 1×105 CFU MTB strain H37Rv. Four weeks later，bacterial load in spleens was determined. RESULTS: The titer of sera specific antibody in BALB/c mice immunized with fusion expression protein MPT64ESAT6 was 1∶1500. The SI of lymphocytes in fusion protein immunized group was 2.23, significantly higher than that of saline immunized group (0.88). The IFNγ level in culture supernatant of spleen lymphocytes from the mice immunized with fusion proteins were (4.28±0.27) μg/L, significantly higher than that of saline immunized group ［(0.48±0.17) μg/L, P
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