nullGel Permeation ChromatographyGel Permeation Chromatography翟光群
2010年3月TOCTOCBackground
Principles
Applications
GPCGPCBackground
From HPLC to GPC
HPLC: interaction chromatography;
GPC ?
GPCGPCBackground
GPC
Preparative GPC
SEC-Size Exclusion Chromatography: Includes rigid stationary phases
GPC-Gel Permeation Chromatography: “Soft” gel stationary phases
GFC-Gel Filtration Chromatography: Separation of biological molecules (nature’s polymers) in an aqueous environment
GPCGPCBackground
GPC
1860~1930:多孔
材料
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具有吸附-分离,尤其是染料,的功能;
1940~1970:一般意义上的液相色谱出现了;
1963: 世界首台商用GPC由Waters公司推出;
1970: HPLC (测试更快、分离效果更好、样品需求量更小、检测器更灵敏);
HPLC的新技术不断地运用到GPC领域中
GPCGPCBackground
GPC
Waters
Shimadzu
Perkin-Elmer
HP-Agilent
Polymer Laboratory
ViscotekGPCGPCprinciples
Structure
GPCGPCprinciples
Structure
GPCGPCprinciples
Structure
GPCGPCprinciples
StructureGPCGPCprinciples
StructureGPCGPCprinciples
StructureGPCGPCprinciples
Structure
Solvent:
Organic THF, CHCl3, DMF
Aqueous
Temperature
Ambient <50 oC
High Temperature <150 oC
Pore size
Tranditional pore size
Mixed Bed columnsGPCGPCprinciples
Conventional CalibrationGPCGPCprinciples
Conventional Calibration
GPCGPCprinciples
Conventional CalibrationGPCGPCprinciples
Universal CalibrationGPCGPCprinciples
Universal Calibration
GPCGPCprinciples
Detector
RI/UV
LLS
Viscometer
GPCGPCprinciples
Detector
RI
GPCGPCprinciples
Detector
RI
GPCGPCprinciples
Detector
RIGPCGPCprinciples
Detector
ViscometerGPCGPCprinciples
Detector
ViscometerGPCGPCprinciples
Detector
MALLS
GPCGPCprinciples
Detector
MALLSGPCGPCprinciples
Detector
MALLSGPCGPCprinciples
Branched PolymerGPCGPCprinciples
Branched PolymerGPCGPCprinciples
Branched Polymer