如何回复审稿人意见(Response-to-Reviews)

如何回复审稿人意见(Response-to-Reviews)如何回复审稿人意见(Response-to-Reviews)PAGE如何回复审稿人意见(Response-to-Reviews)DearEditor,Wehavestudiedthevaluablecommentsfromyou,theassistanteditorandreviewerscarefully,andtriedourbesttorevisethemanuscript.Thepointtopointrespondstothereviewer’scommentsarelistedasfollowing:Respondstothereviewer’scomments:Reviewer1Comment1:inpage3,line40,wefedrats..."changedtoratswerefedwith...Response:Accordingtothereviewer’scomment,wehavecorrectedthesentence.Furthermore,wehavehadthemanuscriptpolishedwithaprofessionalassistanceinwriting.Comment2:page25.Thestyleofreference40isnotright(usinginitialsforthefirstnames).Sincethispaperhasbeenpublished,thevolumeandpageNosshouldbeprovided.Response:Thankyouforyourcarefulwork.Wehaveaddedthevolumeandpagenumbersforreference40.Reviewer2Comment:Iwouldliketothanktheauthorsfortheireffortsinaddressingthecriticismswithadditionalexperiments.Theonecriticismthattheydidnotaddresswasrelatingtoenergyexpenditureasthereasonthattheanimalsonthelowcalciumdietgainedmoreweight.WhileIunderstandthatperformingthisexperimentwillnotaffecttheconclusionofthismanuscript,IdobelievethatthispointcouldbediscussedintheDiscussionsection.Response:Thankyouforyourvaluableadvice.Basedonthepreviousrevision,wefurtheraddresstherelationshipbetweenlowcalciumdietandenergyexpenditureinthesectionofdiscussionaccordingtoyourthoughtfulcomments.Reviewer3Comment1:Inthetextyouoftenwrite:“Aspreviouslydescribed”.Unlessthatpaperisfromyourlaboroneofthemethodpaperco-authorsisonthepresentMSthisisnotquitepropersincethestatementinfersmethoddevelopmentfromyourlab.Therearenumerousinstanceslikethatinthemethodssection;theseshouldallbechanged“accordingtothosedescribedby…..”Response:Wearesorryforthislanguagemistake.Wehavecarefullycorrectedthisphrasethroughoutthemanuscriptaccordingtoyourcomment.Comment2:Therearestillsomewording,sentencestructureandgrammaticalissueseveninthisbasicallywellputtogetherMS.Forexample,whileauthorsmayhavebeenexcitedaboutthedatayoucannotstartasentencewith“Excitedly”inline418or“Whatever”inline395.Response:Thankyouverymuchtopointoutthesentencestructureandgrammaticalissuesinourmanuscript.Accordingtothecommentsfromyouandtheeditors,wepolishedthemanuscriptwithaprofessionalassistanceinwriting,conscientiously.Comment3:Inmyviewabigomissioninthisworkisignoringtheanabolicsideoflipidmetabolismaswellasthermogenesisissues.ForexampleallanimalsconsumedthesameamountoffeedbutwehadextrafatstorageinthelowCadietgroups.SowheredidtheextraenergygoZemeletal(citation34)insimilarworkindicatethatincreasedthermogenesisonthehighCadietexplainsthedissipationofdietaryenergy.FurthereventhoughZemeletal(#34)indicatedlipogenesiswasenhancedinthelowCadietsthatwasin2000andyoushouldhavemonitoredexpressionofFASandUCPeitherasmRNAabundanceoractualFAS/UCPchangesviaproteomicsorblottingtechniques.InanycasethesecontrolsaremissinghereandnotemphasizedintheMS.CasualreadingofthispaperwouldleadtotheconclusionthatthedietaryCaeffectonfatdepositionisstrictlyafunctionofincreasedordecreasedlipolysis.Whilelipolysisappearstobeamajorplayer,lipogenesisandthermogenesiscannotbeignoredforcompleteness.InFig8youalsoshowadeclineincAMPforthelowCadiet.WellbetaagonistsorcAMPenhancersregulatetranscriptionofadiposeandliverFAS(inrats(JBiolChem271:2307,1996)andrecentlywithlargeanimalmodels(HausmanetalJAnimalScience87:1218,2009andHalseyetalJAnimalScience89:1011,2011).InadditioncAMPlevelscouldhavebeenmonitored.IreallydonotlikethelastsentenceintheAbstractline47-50whereyoustatethat“lowcalciumdiet-inducedincreaseinfatmasswasduetoenhancedlipogenesismediatedbyanupregulatedCaSRsignalingpathway”Yourresultshereshownosuchthing,thisisacompletelyfalsestatementbasedondataherein.Correct.YoushowthathighCadietsenhancelipolysisandlowCadietsareantilipolytic.Youdidnotmonitorlipidanabolismhereatall.Seealsoline255-257andlines333-335ofyourMS.Response:Thankyouforyourvaluableandthoughtfulcomments.Asyousuggestedthattheanabolicsideoflipidmetabolismaswellasthermogenesisissuesshouldbemonitored.Wereallyagreewithyourviewpoints.Inthepresentstudy,wedidfindthatlowcalciumdietincreasedthemRNAleveloffattyacidsynthase(FAS)inwhiteadiposetissue.Furthermore,theFASmRNAlevelwerealsoincreasedinadipocytesaftertreatmentwith1,25-(OH)2D3inin-vitroexperiments.However,theincreasedFASmRNAlevelswerenotaffectedbypreventingeitherthenuclearvitaminDreceptor(nVDR)orcalcium-sensingreceptor(CaSR),suggestingthatFASmightnotbeinvolvedintheCaSRpathway.Inaddition,wethoughtthatFASplayeditsroleinfattyacidsynthesismainlyinliverpreviously.Besides,themanuscriptwasrequiredtorestrictnumberoftotalwordsandourpreviousfocuswasontheantilolyticroleofCaSRintheprocessoffataccumulation.SoweignoredtoprovidethedataofFASmRNAlevelsinthesubmittedmanuscript.Inthenewlysubmittedmanuscript,wehaveprovidedthemRNAlevelsaccordingtoyourhelpfulsuggestion.WehavereportedtheeffectsofdietarycalciumonUCP2mRNAlevelsinadiposetissueandUCP3inskeletalmuscleinourpreviousstudiesADDINADDIN(1,2).Thus,webelievedthatlowcalciumdietledtodecreasedthermogenesisinthepresentstudy.Itwasapitythatwedidnotmeasuretheratcoretemperatureinthosestudies.TheUCP2mRNAlevelsinadipocyteswereobservedtobedecreasedaftertreatmentof1,25-(OH)2D3.ThiseffectwaspreventedbyusingnVDRCaSRgenesilencingbutnotbyCaSRgeneknockdown,suggestingthatUCP2wasnotinvolvedinCaSRpathways.Inthenewlysubmittedmanuscript,wehaveprovidedtheUCP2results.Thankyouforyourcarefulreadingofourmanuscript.Weareverysorryforourfaultstatementintheabstract.Wehavecorrecteditinthenewmanuscript.Comment4:ApointthatdoesnotemergewellfromthediscussionishowlowCaintakesresultinhigherintracellular[Ca]concentrationsandreallytheeffectsonfatdepositioninthecellsinmanywaysareduetoanincreasedintracellularCalevelmediatedviaCaSRexpressionincreasesandtheeffectofVitD3onnVDRshowinFig8.TheauthorsmustremindreadersthatCalevelsinthebloodareunderhormonalregulation(Calcitonin,PTHandVitD3).ThuswhendietslowinCaareconsumedandbloodCadecline,PTHandVitD3arecalledupontomobilizeboneCatoreplenishthebloodCa.ThencoupledwithanincreaseinCaSRmoreCaactuallyisfoundinATdespitethefactthatmanywouldthinktheATCalevelshoulddecline.Thereasonisthattissue/circulatingCalevelsarenotdietdependedbutregulated.ThevastbonestoresofCawillprovideampleCahereespeciallyduringastudyofthislength.Whileauthorsaddresstheseissuesmaybecouldbepresentedinalesscomplicateddiscussion.Response:Thankyouforyourinstructivesuggestions.WearesorryfornotdescribingtheeffectoflowcalciumdietonintracellularcalciumconcentrationsmediatedbyCaSR,aswellastheimpactofhormoneregulationonserumcalciumlevelsclearly.Accordingtoyourhelpfuladvice,wehaverewrittenthesetwopartsinthesectionofdiscussion.Thankyouagain.Comment5:NotallcitationsareinJNstyleResponse:WehavecarefulrecheckandcorrectedthestyleofthecitationsaccordingtotherequirementofJN.Comment6:Abstractconclusiondiffersfromlines255-257and333-335;WHY?Response:Thankyouforyourcarefulreadingofourmanuscript.Theconclusionfromlines255-257isabouttheeffectoflowcalciumdietonserumlevelsoffreefattyacids(FFAs)andlipids.WeconsideredFFAandglycerolasindicatorsofTGhydrolysisinadiposetissue.ThelowcalciumdietcauseddecreasedserumFFAandglycerollevelswithoutinfluencinglipoproteinlipase(LPL)activity,sowethoughtthelipolyticeffectofadiposetissuetobesuppressedbylowcalciumdiet.Theconclusionfromlines333-335wasabouttheeffectof1,25-(OH)2D3whoselevelswereincreasedunderlowcalciumconditionsonlipolysis.WeusedtheglycerollevelastheindicatorofTGhydrolysisinadipocytes.Boththeinvivoandinvitroexperimentsshowedlowcalciumstatuscausedanantilipolyticeffect.Comment7:Line150-153.TheqRT-PCRmethodologyisnotatallunderstandableasyouciteaTexasA&Mpublishedpaper.ThisiscompletelyinsufficientwiththenewlyestablishedstandardsongeneexpressionviaqRT-PCR.Thereisnomentionofefficienciesofamplificationsinthesedatanorhowtheuseofthereferencegenewasestablishedetc.IthinkPfafflandBustinhaverecentlywrittenanarticleonthis;pleasetotallyrevise150-153inlinewithwhatyoudidandapplyingthenewstandards.Response:Thankyouverymuch.BecausetheJNrestrictsthenumberoftotalwordsofmanuscript,wecitedtheTexasA&Mpublishedpaper.Inthenewlysubmittedmanuscript,wedescribethedetailedprotocolsinourlab.Comment8:Line179onNotclearasinsentencestalkaboutdifferentATcellsourcesetc..revise.Response:Wearesorryfornotaddressingtheadiposetissuecellsourcesclearly.Wehaverewrittenthemethods.Comment9:AnypreviousdocumentableworkwithsiRNAResponse:Yes,wehavedocumentableworkwithsiRNAinourresearchteam.TheresultswerepublishedinthejournalofBiochemBiophysResCommunADDINADDIN(3).Comment10:Line214..CulturedprimaryratadipocytesandSW872adipocytes……Response:Thankyouverymuch.Accordingtoyourcomment,wehavehadthemanuscriptpolishedandcorrectedthemistakes.