送检样品抑菌实验报告
检测报告
检测项目: 抗生素抑菌实验
送检日期: 2012-09- 10
送检单位:
检 验 员: 周俊
1 实验材料与方法
1(1 实验材料
Ke station a level 75275 Simon station two level 11333 South door station level 16650 Creek station two level 6000 gold hung station two level 15000 Pro Hong Kong station level 64870 Figure 3.1-1 Yibin city main highway passenger station distribution schematic (4) Highway freight station distribution according to information displayed, Yibin City status only has 2 a three level LTL Terminal, respectively for nine home village Terminal and
指示菌、TSB肉汤、麦氏比浊管、LB平板培养基、MRS肉汤、L型波棒、牛津杯、灭菌陶瓷盖、灭菌试管、微量移液器、密封发酵管、磷酸缓冲液等。 2(1 实验方法
2.1.1 指示菌
使用大肠杆菌O157,O139,K88,K99型、金色葡萄球菌与沙门氏菌。 2.1.2 指示菌菌悬液的准备
(1)细菌复苏:菌种平板划线,36?培养20h;挑取单菌落于TSB肉汤,置37?振荡培养16h;接种TSB肉汤,置37?振荡培养16h。
(2)采用直接菌落悬液法,将培养好的菌液调整到5个麦氏浓度(也就是细菌数
8 为1×10cfu/ml)。此时指示菌菌悬液制备完毕。(注:在调整过程中需使用PH 7.2的磷酸缓冲液进行菌液的麦氏浓度调整)
2.1.5 LB检测平板的制备的准备
将LB肉汤培养基加入琼脂后,溶解均匀,在高压灭菌釜中。121?,高温灭菌20min。将已灭菌的琼脂培养基倒在玻璃培养皿内,每皿15ml(下层),待其凝固。再继续将灭好的LB琼脂培养基再次加固在已凝固的培养基上待凝固(上层)。使LB平板增厚。凝固后并使培养皿中水份干燥透,取100μl的指示菌菌悬液在平板上用L型波棒均匀涂布,涂布后以平板无可见水滴为准,此时的平板立刻进行抑菌试验。
2.1.6 样品原粉在培养基上的放置
精确秤取样品0.05克轻轻放入培养皿中,在水平放置的平皿中均匀地放置。 2.1.7 样品抑菌扩散及指示菌的培养
将加入样品原粉的平皿放置37?的恒温培养箱内,培养16h~24h后,进行拍照记录。
结果:
图中黄褐色褐色粉末为15%杆菌肽锌。
O157 O139 Ke station a level 75275 Simon station two level 11333 South door station level 16650 Creek station two level 6000 gold hung station two level 15000 Pro Hong Kong station level 64870 Figure 3.1-1 Yibin city main highway passenger station distribution schematic (4) Highway freight station distribution according to information displayed, Yibin City status only has 2 a three level LTL Terminal, respectively for nine home village Terminal and
K88 K99
金葡 沙门
抑菌圈大小如下
表
关于同志近三年现实表现材料材料类招标技术评分表图表与交易pdf视力表打印pdf用图表说话 pdf
:(单位mm)
金葡 沙门 O157 O139 K88 K99
Ke station a level 75275 Simon station two level 11333 South door station level 16650 Creek station two level 6000 gold hung station two level 15000 Pro Hong Kong station level 64870 Figure 3.1-1 Yibin city main highway passenger station distribution schematic (4) Highway freight station distribution according to information displayed, Yibin City status only has 2 a three level LTL Terminal, respectively for nine home village Terminal and
15% 杆菌肽锌 22 23 24 21 28 23
根据
标准
excel标准偏差excel标准偏差函数exl标准差函数国标检验抽样标准表免费下载红头文件格式标准下载
,当抑菌圈大于15mm时,该药对细菌敏感。
结论:
结果表明,该杆菌肽锌抑菌圈均大于15mm,对O157、O139、K88、K99、沙门氏菌、金色葡萄球菌均敏感,具有好的抑菌作用,可以选用。
温馨提示:本次实验结果只对所送的样品负责~
Ke station a level 75275 Simon station two level 11333 South door station level 16650 Creek station two level 6000 gold hung station two level 15000 Pro Hong Kong station level 64870 Figure 3.1-1 Yibin city main highway passenger station distribution schematic (4) Highway freight station distribution according to information displayed, Yibin City status only has 2 a three level LTL Terminal, respectively for nine home village Terminal and