测序通用引物

测序通用引物 载体名称 上游引物(5'primer) 下游引物(3'primer) P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152) pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGH rev pAcG2T pGEX-5 not available pACT T7EEV T3/EBVrev pACT2 GAL4 ADfor pGAL4-ADrv pACT2 p17110 p12584 pACYC184(BamHI-site) PBRforBam PBRrevBam pACYCDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1 pACYCDuet-1(MCSII) DuetUp2 T7-Terminator pAdEasy-1 not available not available pAdTrack-CMV not available not available pAS2-1 GAL4 Bdfor/P24990 pGAL4-BDrv/P31430 pB42AD pB42ADF PB42ADR pBABE pBMN_5' pBacPAK8 BAC1 BAC2 pBAD pBAD-F pBAD-R pBAD/HisABC pBAD-5' pBBR1MCS T3/M13rev T7/M13for pBD-GAL4 pBD-GAL4-F PBD-GAL4-R pBI121 35S NOS pBIND GAL4-Bdfor T3,EBVrev pBK-CMV M13F/T7 M13R/T3 pBluescriptIIKS(+/-) T3/M13rev T7/M13for(优先用M13F) pBluescriptIISK(+/-) T3/M13rev T7/M13for pBluescriptKS(+/-) T3/M13rev T7/M13for pBluescriptSK(+/-) T3/M13rev T7/M13for pBR322(BamHI-site) PBRforBam PBRrevBam pBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHind pBR325(BamHI-site) PBRforBam PBRrevBam pBR329(BamHI-site) PBRforBam PBRrevBam pBridge(MCSI) GAL4-Bdfor 3'BD pBridge(MCSII) not available not available pBS-T II T3/M13rev T7/M13for pBS(AMP) T7 T3 pBSR T3 T7ter pBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrev pBudCE4.1(MCSII) EF-1α Fwd BGH pBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrev pBV220 pBV220F PBV220R pCAL-c T7/Tac-Profor T7-Terminator pCAL-n T7/Tac-Profor T7-Terminator pCAMBIA1301(1300) pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII) pCAMBIA1304 pCAMBIA1301-5'(ECORI) pCAMBIA1301-3'(HindIII) pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49) pCANTB5E S1 S6 pCAT3-enhancer RVP3 此载体无反向引物 pCDFDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1 pCDFDuet-1(MCSII) DuetUP2 T7TER pcDNA1.1 CMV-Profor,T7 pCDM8-3 pcDNA2.1 M13for/T7 M13rev/Tac-Profor pcDNA3 T7/CMV-Profor BGH rev/SP6 pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGH rev pcDNA3.1/His(_A,_B,_C) T7/CMV-Profor BGH rev pcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGH rev/c-mycrev pcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor BGH rev/V5rev pcDNA3.1/Hygro(+,-) T7/CMV-Profor BGH rev/V5rev pcDNA3.1/Zeo(+.-) T7 BGHrev pcDNA3.3TOPOTA CMV-Profor TKPAreverse pcDNA4/HisMax(_A,_B,_C) Xpressfor BGH rev pcDNA4/HisMax-TOPO Xpressfor BGH rev pcDNA4/HisMax-TOPO/lacZ Xpressfor BGH rev pcDNA4/myc-His(_A,_B,_C) T7,CMV-Profor BGH rev,c-mycrev pcDNA4/TO TREfor/CMV-Profor BGH rev pcDNA4/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pcDNA4-TET/ON/AMP+ CMV-Profor pcDNA5/FRT T7/CMV-Profor BGH rev pcDNA6/myc-His(_A,_B,_C) CMV-Profor,T7 BGH rev,c-mycrev pcDNA6/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pCEP4 pCEP-F/CMV-Profor EBVrev pCI T7(17BASE) EBVrev pCI-neo T7(17BASE) EBVrev,T3 pCMS-EGFP T7 T3 pCMS-EGFP T7 EBVrev,T3 pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F PCMV5R pCMV6 CMV-Profor pCMV6-3、HGHrev pCMV6-XL4 CMV-Profor/T7 pCMV6-3、HGHrev pCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrev pCMV-MYC(AMP+) PCMV-F PCMV-R, EBVrev pCMVmyc/nuc CMV-Profor BGH rev,c-mycrev pcmv-scriptEXVector T3 T7 pCMV-Sport6 T7/M13for SP6/M13rev pCOLADuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1 pCOLADuet-1(MCSII) DuetUP2 T7TER pCR2.1 T7,M13for M13rev pCR2.1-TOPO M13for,T7 M13rev pCR3.1 T7 BGH rev pCR3.1-Uni T7 BGH rev pCR4 T3/M13rev T7/M13for pCR4-Blunt T3/M13rev T7/M13for pCR4-TOPO M13rev,T3 M13for,T7 pCR-Blunt M13rev T7,M13for pCR-BluntII M13rev/SP6 T7/M13for pCR-BluntII-TOPO M13rev/SP6 M13for/T7 pCRII M13rev/SP6 T7/M13for pCRII-TOPO M13rev/SP6 M13for/T7 pCR-ScriptSK(+) T7/M13for T3/M13rev pCR-XL-TOPO M13rev T7,M13for pCS2+(MCSI) SP6 EBVrev,T7? pCS2+(MCSII) T3 pSG5-3 pDB_GAL4cam T7P pDB_leu PDBLeu-5 PDBLeu-3 pDEST22 DEST22F DEST22R pDEST15 pGEX-5 T7-Terminator pDEST20 pGEX-5 pFastBac-3 pDEST26 TREfor/CMV-Profor T7/M13for pDisplay T7/CMV-Profor c-mycrev pDNR-LIB(MCS_A) M13for,T7 pSG5-3,M13R pDonar M13F M13R pDONR201 PDONR-F PDONR-R pDONR207 PDONR-F PDONR-R pDONR223 M13F M13R pDrive T7/M13rev SP6/M13for pDsRED1-C1 (DsRed1-N-f) DsRed1-C-r pDsRed1-N1 CMV-Profor RFP-Nrev,CMV-R? pDsRed2-N1 (DsRed1-N-f) DsRed1-C-r pDsRed2-C1 (DsRed1-N-f) DsRed1-C-r pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’ pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-R pDSRED-monomer-N1(KAN+) dsRed1_N_primer pDSRED-N1(KAN+) pEGFP-N-5’ pDSRED-N-R pEASY-Blunt M13F T7/ M13R T7/ M13R(距离插入位点合适，pEASY-BluntSimple M13F 优先安排) M13R(距离插入位点合适，优pEASY-T1 Simple M13F 先安排) pEASY-T1 M13F/T7 M13R pEASY-T3 M13F/T7 M13R/SP6 pECFP-N(C) PECFP-N-5. PECFP-N-3, pEF/myc/cyto/ER/mito/nuc pEF-5 Pcdna3.1R/BGH rev pEF1(4,6/myc-His) T7/pEF-5 Pcdna3.1R/BGH rev pEF1(4,6/V5-His) T7/pEF-5 Pcdna3.1R/BGH rev pEF1(4;6/His_A;_B;_C) T7/Xpressfor Pcdna3.1R/BGH rev pEF5/FRT/V5-D(-TOPO) T7/pEF-5 Pcdna3.1R/BGH rev pEGFP M13rev EGFP-Nrev pEGFP-1 not available EGFP-Nrev/ PEGFP-N-3′ pEGFP-C PEGFP-C-5′ PEGFP-C-3′ pEGFP-C1 EGFP-Cfor SV40-pArev pEGFP-C2 EGFP-Cfor SV40-pArev pEGFP-C3 EGFP-Cfor SV40-pArev pEGFP-F EGFP-Cfor SV40-pArev pEGFP-N PEGFP-N-5′ PEGFP-N-3′ pEGFP-N1 CMV-Profor EGFP-Nrev pEGFP-N2 CMV-Profor EGFP-Nrev pEGFP-N3 CMV-Profor EGFP-Nrev pENTR M13F M13R pET-11(-a,-b,-c,-d) T7 T7-Terminator pET-12(-a,-b,-c) T7 T7-Terminator pET-14b T7 T7-Terminator pET-15b T7 T7-Terminator pET-16b T7 T7-Terminator pET-17b T7 T7-Terminator pET-17xb T7 T7-Terminator pET-19b T7 T7-Terminator pET-20b(+) T7 T7-Terminator pET-21(-a,-b,-c,-d)(+) T7 T7-Terminator pET-22b(+) T7 T7-Terminator pET-23(-a,-b,-c,-d)(+) T7 T7-Terminator pET-24(-a,-b,-c,-d)(+) T7 T7-Terminator pET-25b(+) T7 T7-Terminator pET-26b(+) T7 T7-Terminator pET-27b(+) T7 T7-Terminator pET-28a(-b,-c)(+) T7 T7-Terminator pET-29a(-b,-c)(+) S.tag/T7 T7-Terminator pET-3(-a,-b,-c,-d) T7 T7-Terminator pET-30_Ek/LIC T7 T7-Terminator pET-30_Xa/LIC T7 T7-Terminator pET-30a(-b,-c)(+) S.tag/T7 T7-Terminator pET-31b(+) not available T7-Terminator pET-32_Ek/LIC TRXfor T7-Terminator pET-32_XA/LIC TRXfor T7-Terminator pET-32a(-b,-c)(+) S.tag/TRXfor T7-Terminator pET-33b(+) T7 T7-Terminator pET-34b(+) S.tag T7-Terminator pET-35b(+) S.tag T7-Terminator pET-36b(+) S.tag T7-Terminator pET-37b(+) S.tag T7-Terminator pET-38b(+) not available T7-Terminator pET-39b(+) S.tag T7-Terminator pET-40b(+) S.tag T7-Terminator pET-41_Ek/LIC GST-Cfor T7-Terminator pET-41a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-42a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-43.1a(-b,-c)(+) S.tag coliDOWN pET-43.1a_EK/LIC S.tag not available pET-44a(-b,-c)(+) S.tag T7-Terminator pET-44a_EK/LIC S.tag not available pET-45b(+) T7 T7-Terminator pET-46_Ek/LIC T7 T7-Terminator pET-5(-a,-b,-c) T7 T7-Terminator pET-52b(+) T7 T7-Terminator pET-7 T7 T7-Terminator pET-9(-a,-b,-c,-d) T7 T7-Terminator pETcoco-1 T7 T7-Terminator pETDuet-1(MCSI) pETUP1 DuetDOWN1 pETDuet-1(MCSII) DuetUp2 T7-Terminator pET-T4 T7 T7-Terminator pEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’,CMV-Profor pEGFP-N-3’ pFLAG-ATS N26for pTrcHis-rv;C24rev pFLAG-CTC N26for pTrcHis-rv;C24rev pFLAG1 N26for pTrcHis-rv;C24rev pFLAG-CMV(-2) CMV-30;CMV-Profor CMV-24 pFLAG-CMV1 CMV-30 CMV-24 pGAD424 GAL4_ADfor 3'AD pGADGH GAL4_ADfor 3'AD pGADT7 T7/GAL4_ADfor 3'AD pGADT7-Rec T7/GAL4_ADfor 3'AD pGADT-T T7 3,BD pGAD10 GAL4_ADfor 3'AD pGAPZa-A a-FACTOR 3'AOX pGBKT7 T7 3,BD MATCHMAKER5'DNA-BD/GALpGBT9 4BD MATCHMAKER3'DNA-BD pGEM-1 T7 SP6 pGEM-3 T7 SP6 pGEM-3Zf(+)(-) SP6/M13rev T7/M13for pGEM-4 T7 SP6 pGEM-4Z T7/M13for SP6/M13rev pGEM-5Zf(+)(-) SP6/M13rev T7/M13for pGEM-7Zf(+)(-) SP6/M13rev T7/M13for pGEM-9Zf(+)(-) T7/M13for SP6/M13rev pGEM-T T7/M13for SP6/M13rev pGEM-T_Easy T7/M13for SP6/M13rev pGene/V5-His_A(_B,_C) pGENE-5 BGH rev,V5rev pGEX-2T pGEX-5 pGEX-3 pGEX-2TK pGEX-5 pGEX-3 pGEX-3X pGEX-5 pGEX-3 pGEX-4T-1(-2,-3) pGEX-5 pGEX-3 pGEX-5X-1(-2,-3) pGEX-5 pGEX-3 pGEX-6P-1(-2,-3) pGEX-5 pGEX-3 pGL2 GLP1 GLP2 GLP2(RVP4)(没有特别说明用pGL3 RVP3 GLP2) pGL3-Basic(MCSI) RVP3(PGL3+)? GLP2(PGL3-)? pGL3-Basic(MCSII) RVP4, PLXSN-3'? pGL4 pGlow(-TOPO) T7 pGlow-3 pGM-T T7 SP6 pGWC SP6 T7 pHook-2 T7/CMV-Profor not available pIND/V5-His_A(_B,_C) not available BGH rev,V5rev PinpointTMVector PinpointPrimer SP6 pIRES(MCSI) T7 pIRES-3 pIRES(MCSII) not available T3,EBVrev pIRES2-EGFP CMV-Profor,pIRES2-EGFP.P5’ IRESrev,pIRES2-EGFP.P3’ pIRES-hrGFP-2a CMV-Profor,T3 pIRES-hrGFP-3 pIRESneo2 T7/CMV-Profor pIRES-3 pIVEX(all) T7/pBRrevBam T7-Terminator pLEXA pLEXA-F pLEXA-R pLNCX pLNCX-F pLNCX-R pLNCX2 CMV-Profor pRevTRE-3 pLVTHM(H1启动子下游) H1primer SP6 pLXSN pLXSN-F pLXSN-R pMALc MalE primer M13F(-47) pMAL-c2E MalE primer M13F(-47) pMAL-C2x Pmal-c2x-F(P5’) Pmal-C2X-R(P3’ ) pMALd MalE primer M13F(-47) pMALe MalE primer M13F(-47) pMAL-P4X MalE primer M13F(-47) pMAL-p2X MalE primer M13F(-47) pMET A/B/C AUG1F AUG1R pMD18-T M13R(-48)ECORI M13F(-47)(HIND?) pMD19-T M13F47 M13R48 pMD20-T M13F47 M13R48 pMOSBlue T7/M13rev M13for pMSCV-puro pLXSNfw pMSCVrev pMT/V5-His_A(_B,_C) Mtfor BGH rev,V5rev pNTAP T3 T7 pCTAP pCTAP5'primer T7 pOTB7 M13rev/T7 M13for/SP6 pOptiVEC-TOPO TA CMV-F/CMV-Profor EMCVIRES reverse pPC86 PPC86-F PPC86-R pPC97 PBD-GAL4-F PBD-GAL4-R pPIC3.5K 5'AOX 3'AOX pPic9k(A+,Z+) 5’AOX(Ppic9k-f)/a-factor 3’AOX(Ppic9k-R) pPICZa 5'AOX/PPICZa-F 3'AOX pPROEXHTA M13R(-48) 无反向引物 pProEX-HTa(b,c) M13R48/ Tac-Profor pBADrv/pTrcHis-3 pPD129.36 M13F20/M13F pQE30or40or60(AMP+) PQE30-F PQE-30R pRC/CMV2/CAT CMV/T7 pRECEIVER CMV-F 此载体 无反向引物 pRECEIVER-M03 pRECEIVER-M03F pRECEIVER-M03R pREP4 pREP4 forward primer EBV reverse primer pRK5 SP6 pSG5-3 pRSET T7/Xpressfor T7ter pRSFDuet-1(MCSI) DuetUP1 DuetDOWN1 pRSFDuet-1(MCSII) DuetUP2 T7TER pSecTac2 T7/CMV-Profor BGH rev/c-mycrev pSG5 pSG5-5,/T7 PSG5-R(SV40) pSHlox1 T7 SP6 pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSI T7 EBVrev pSilencer1.0-U6 T7 T3 pSilencer2.0-U6 T7 M13for pSilencer2.1-U6neo T7 M13for pSilencer3.1-H1hygro M13F(-47) 3.0REV pSilencer4_1-CMVneo pSilener4.1-5' CMV-Profor pSILENCEV2.0-U6 T7 2.0REV pSIneo T7 EBVrev,T3 Psit T7 T7T pSK01-T M13F M13R pSK-CMV(KAN+) T7 T3 pSOS Psos5' Psos3' pSP64 SP6 M13rev pSP64/65 SP6 此载体无反向引物 pSP65 SP6 M13rev pSP70 SP6 T7 pSP71 SP6 T7 pSP72 SP6 T7 pSP73 SP6 T7 pSport1 SP6/M13F(-47) T7/M13R pSPORT1 SP6/M13for T7/M13rev pSPT18 SP6 T7 pSTBlue T7/M13rev SP6/M13for pSUPER.Basic T7/M13F M13R pSUPER T7 T3 pSURE-T M13F M13R pSURE-Tsimple M13F-47 M13R-48 pSuperCos pBRforEco T7 pT3T7 M13rev/T3 M13for/T7 pT7-7 T7 not available pT7Blue? T7 M13F(-47) pT7T3_18U M13rev/T7 M13for/T3 pT7T3D-PAC M13rev/T7 M13for/T3 pTA2 M13F/T7(优先使用M13F) T3/M13R pT-Adv M13rev T7,M13for pTAg T7/M13rev M13for pTARGETTM pTarget.F(在T7前面)/T7 pTarget.R pTG19-T M13F(-47) M13R(-48) pTARGETTM T7/M13F PTARGET-SEQ pThioHisA,B,andC Trx Forward Trx Reverse / M13for pTO-T7 T7 T7TER pTRC99A PTRC99A-F pTrcHis-rv pTrc99a-c PTRC99C-F PTRC99C-R pTRC99a-c PTRC99C-F PTRC99C-R pTrcHisB PTrcHisA-F/Xpressfor PTrcHisA-R pTrcHisA PTrcHisA-F PTrcHisA-R pT-REX-DEST30 TREfor T7 pTriEx1 T7/TriExup TriExdown pTriEx1.1 T7/TriExup TriExdown pTriEx1.1_Hygro T7/TriExup not available pTriEx1.1_Neo T7/TriExup IRESrev pTriplEX1 TriplEx-LD-5 T7,TriplEx-LD-3 pTriplEX2 TriplEx-LD-5;pTR5‘ T7,TriplEx-LD-3 pTWIN-1 T7 T7TER pTXB1 T7 MxeInteinrev pTXB2 T7 MxeInteinrev pTXB3 T7 MxeInteinrev pTYB1 T7 Inteinrev pTYB2 T7 Inteinrev pTYB3 T7 Inteinrev pTYB4 T7 Inteinrev pTZ_18U T7/M13rev M13for pUB6/V5-His UB-F BGH rev,V5rev pUC13 M13rev/M13rev(-48) M13for/M13for(-49) pUC18 M13rev/M13rev(-48) M13for/M13for(-49) pUC18(19)/118(119) M13F(-47) M13R(-48)/PUC19(HIND?) pUC19 M13rev/M13rev(-48) M13for/M13for(-49) pUC57 M13rev/M13rev(-48) M13for/M13for(-49) pUC8 M13rev/M13rev(-48) M13for/M13for(-49) pUC9 M13rev/M13rev(-48) M13for/M13for(-49) pUM-T M13F(-20) M13R(-20) pUCm-T(AMP+) M13F/T7 M13R pUNI/V5-His not available BGH rev,V5rev pVAX1T7 T7/CMV-Profor PCDNA3.1R/BGHR pVP22/mycHis VP22-Cfor BGH rev,c-mycrev pVP22/mycHis2 T7/CMV-Profor VP22-Nrev pWE15 pBRrevHind T7 pYES2 T7 pYes2.R pYES-DEST52 T7/GAL1-Profor V5rev/CYC1-Terminator pYEX_4T_1 Clontech pYEX_4T-3 pYEX_4T_2 pGEX-5 pYEX_4T-4 pYEX_4T_3 pGEX-5 pYEX_4T-4 pYEX_4T_4 pGEX-5 pYEX_4T-4 pZeoSV2+/hu-Tyr T7 not available pZERO-1 M13rev/SP6 T7/M13for pZERO-2 M13rev/SP6 M13for/T7 pZome-1-C Zome-F Zome-R pZome-1-N Zome-F Zome-R YCp50(BamHI-site) PBRforBam PBRrevBam YEp13(BamHI-site) PBRforBam PBRrevBam YEp24(BamHI-site) PBRforBam PBRrevBam YIp5(BamHI-site) PBRforBam PBRrevBam RNAi-ReadypSIREN-RetroQ-U6Zs Green U6 菌PCDB T7 BGH 天为时代TA载体 T7 T3 m13(+) T7, T3, pMSCV-neo pMSCV-5' pMSCV-3'