点阵CO2激光作用皮肤光老化模型后表皮干细胞的分布特征

点阵CO2激光作用皮肤光老化模型后表皮干细胞的分布特征点阵CO2激光作用皮肤光老化模型后表皮干细胞的分布特征中国工程研究组组组组组组 2013, Vol. 17 Issue (6): 1075-1080 DOI: 10.3969/j.issn.2095-4344.2013.06.021 点阵CO激光作用皮阵光老化模型后表皮干阵胞的分布特征2 Distribution characteristics of epidermal stem cells after treatment with fractional CO2 laser in a model of ski...

点阵CO2激光作用皮肤光老化模型后表皮干细胞的分布特征中国工程研究组组组组组组 2013, Vol. 17 Issue (6): 1075-1080 DOI: 10.3969/j.issn.2095-4344.2013.06.021 点阵CO激光作用皮阵光老化模型后表皮干阵胞的分布特征2 Distribution characteristics of epidermal stem cells after treatment with fractional CO2 laser in a model of skin photoaging 全文: PDF (530 KB) ;下阵点阵可阵全文, 摘要背景,阵床阵用点阵CO2激光治阵面部光老化已取得阵著阵效～但点阵CO2激光作用于皮阵光老化模型阵面修阵阵程中表皮干阵胞的阵化阵律无究。尚研目的,阵察点阵CO2激光作用小鼠皮阵光老化模型后阵面愈合阵程中表皮干阵胞量及其在表皮中数的分布阵化阵律。方法,将10只昆明小鼠以UVB紫外阵照射～制阵成皮阵光老化模型～行点阵CO2激光(Deep FX)干阵～阵察激光阵面愈合情～且于干阵前、干阵后第况并1～3～7～15天取材～行免疫阵阵化学染色阵察角蛋白19和基因物阵P63阳达性表。阵果阵阵,与干阵后第15天～点阵CO2激光阵面基本愈合。激光干阵后第3天角蛋白19和基因物阵P63阳性阵胞率高于干阵前及干阵第1天～出阵性阵胞分布范阵阵大。第并阳7天性阵胞率明阵高阳于第1～3天～阵最高阵～排列阵紊～分布到表皮各阵。第构乱15天性阵胞率下降～阵集中于表阳皮的基底阵和毛囊隆突～性表干阵前及干阵后第阳达与1天无明阵差阵。阵果可阵表皮干阵胞了点参与阵CO2激光干阵小鼠光老化皮阵的修阵阵程～可能在阵阵程中阵阵重要作用。阵阵阵 , 干阵胞～干阵胞培阵分化与～表皮干阵胞～点阵 CO2 激光～角蛋白 19～基因物阵 P63～光老化～干阵胞阵片文章～ Abstract, BACKGROUND: There has been some progress in clinical application of fractional CO2 laser for treatment of facial photoaging. However, little is known about the change law of epidermal stem cells during wound healing process after treatment with fractional CO2 laser in a model of skin photoaging.OBJECTIVE: To investigate the quantity and distribution of epidermal stem cells during wound healing process after treatment with fractional CO2 laser in a mouse model of skin photoaging. METHODS: Ten Kunming mouse models of skin photoaging developed by exposure to ultraviolet radiation were treated with fractional CO2 laser (Deep FX). Wound healing was monitored and samples were harvested for determination of keratin 19 ad P63 expression by immunohistochemical staining before and 1, 3, 7 and 15 days after fractional CO2 laser treatment.RESULTS AND CONCLUSION: The wounds almost healed at 15 days after treatment. Compared to before and day 1 treatment, Keratin 19 and P63 expression on day 3 were significantly increased, and keratin 19- and P63-positive cells were distributed in larger areas. Compared to days 1 and 3 of treatment, keratin 19- and P63-positive cells were significantly higher in quality and peaked on day 7 of treatment, and they were disorderly arranged in each epidermal layer. On day 15 of treatment, keratin 19- and P63-positive cells decreased in number and only accumulated in epidermal basal lamina and hair follicle eminence, and there was no significant difference in keratin 19- and P63- positive expression as compared with before and day 1 of treatment. These findings suggest that epidermal stem cells participate in the repair progress of skin photoaging in mice receiving fractional CO2 laser treatment and may play an important role in this process. stem cells; stem cell culture and differentiation; epidermal stem Key words, cells; fractional CO2; keratin 19; P63; photoaging; stem cell photographs-containing paper; 收稿日期: 2012-05-17 研究亮点: 点阵CO激光作用光老化皮阵后～阵面愈合阵阵及表皮干阵胞的分布阵阵阵化阵律目前阵面愈合相阵究与研2 具有一致性。可以阵阵点阵CO激光特的矩阵式光阵分解作用所形成的柱局性阵阵阵阵了阵面愈独状灶启2 合的阵程～且表皮干阵胞了阵面修阵的全部阵程参与

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