阿仑膦酸钠论文：载阿仑膦酸钠超高分子量聚乙烯球盘摩擦学性能研究

阿仑膦酸钠 论文 政研论文下载论文大学下载论文大学下载关于长拳的论文浙大论文封面下载 ：载阿仑膦酸钠超高分子量聚乙烯球盘摩擦学性能研究 阿仑膦酸钠论文:载阿仑膦酸钠超高分子量聚乙烯球盘摩擦学性能研究 【中文摘要】超高分子量聚乙烯(UHMWPE)具有良好耐磨性以及优异的生物相容性,已作为人工关节组件之一成功地应用于临床。但是人工关节假体磨损产生的UHMWPE磨屑能诱发假体周围的骨溶解,导致假体松动,成为影响人工关节服役寿命的主要原因之一。本课题组前期将UHMWPE作为药物载体,分别载入防治骨溶解的药物雌二醇(E2)与阿仑膦酸钠(ALN),研制出防治骨溶解的载药UHMWPE(UHMWPE-E2、UHMWPE-ALN)。研究结果显示UHMWPE-E2与UHMWPE-ALN具有满足人工髋关节所需的材料学性能;UHMWPE-E2与UHMWPE-ALN磨屑的稳定释放率分别为40%与34%;体外细胞试验结果表明,载药磨屑释放的药物有抑制骨溶解的作用。但是,由于UHMWPE植入人体后,在承受人体重量的同时,还要保证关节的活动性,因此UHMWPE的摩擦学性能决定人工关节的使用舒适度及服役寿命。另外,关节面承载的压力、相对运动产生摩擦力,材料的磨损机理以及体液的润滑环境将协同对载药UHMWPE的药物释放产生影响。因此载入药物对UHMWPE摩擦学性能的影响以及摩擦工况对药物释放的影响有待进一步研究。本研究采用溶剂挥发法和热压成型法制备载不同浓度UHMWPE-ALN (0.25、0.5和1.0 wt%)。通过傅里叶红外光谱 分析 定性数据统计分析pdf销售业绩分析模板建筑结构震害分析销售进度分析表京东商城竞争战略分析 (FTIR)与热分析(TA)测定药物的热稳定性。对热压成型后的UHMWPE-ALN特征官能团、晶体结构、结晶度、接触角、力学性能进行测定。采用25 vo1%胎牛血清或去离子 水润滑,以氧化锆作为对磨副进行球盘摩擦试验,通过比较不同浓度UHMWPE-ALN与UHMWPE的摩擦系数、磨痕体积以及微观形貌评价ALN对UHMWPE摩擦学性能的影响。采用二次电子(SE)、背散射电子(BSE)及X射线能谱(EDX),分析ALN在UHMWPE-ALN中的分布以及对UHMWPE-ALN磨损的影响。初步研究了加入表面活性剂改善ALN在UHMWPE-ALN中的分散性后的摩擦学性能。采用往复摩擦方式,在去离子水的润滑条件下,研究UHMWPE-ALN1.0 wt%在摩擦过程中的体外药物释放。傅里叶红外光谱(FTIR)与热分析(TA)结果表明,热压过程对UHMWPE-ALN中ALN的官能团没有显著影响,在热压温度范围内ALN没有分解吸热峰;X射线衍射分析(XRD)表明UHMWPE-ALN与UHMWPE具有相似的晶体结构;差示量热扫描(DSC)法和接触角的结果表明,随着载药浓度的增大,UHMWPE-ALN结晶度减小,并且表面趋于亲水;硬度测试表明UHMWPE-ALN的表面硬度没有受到ALN的影响,与UHMWPE相似。由拉伸力学性能测试、小冲杆测试以及纳米划痕测试结果显示当载ALN浓度为0.25 wt%与0.5 wt%时,其力学性能以及抗划伤能力与UHMWPE相似;当载ALN浓度为1.0 wt%, UHMWPE-ALN的力学性能以及抗划伤能力有所下降,尤其是韧性。摩擦试验结果表明,在同种润滑条件以及相同载荷作用下,UHMWPE-ALN 0.25、0.5 wt%的摩擦系数、磨痕体积以及微观形貌与UHMWPE没有显著性差异。而UHMWPE-ALN 1.0 wt%的摩擦系数与磨痕体积有显著增加,微观形貌出现大量松散的无定向纤维,与UHMWPE表面的片状磨损有显著差异。BSE-SEM观察发现在UHMWPE-ALN 1.0 wt%表面有ALN团聚的出现,其耐磨性能的降低与 ALN团聚有关。加入表面活性剂后的UHMWPE-ALN 1.0 wt%的摩擦系数、 磨损体积与UHMWPE无显著性差异,在其磨损形貌中没有观察到由ALN 团聚所引起的大量无定向的纤维,说明ALN的分散性得到改善。摩擦 过程中对UHMWPE-ALN 1.0wt%体外释放表明,在磨损的前200小时,润 滑液中ALN的质量随时间增加而缓慢增大,ALN浓度在0.79-1.16 mol/L之间;200小时到310小时,ALN的释放速度加快,润滑液中ALN 浓度显著增加,在3.66-2.33 mol/L之间。而空白对照组的浸泡液中 没有可测定的ALN浓度。结果表明摩擦工况对ALN的释放有促进作用, 其释放机理还有待进一步研究。本论文结果显示一定载药浓度(低于 0.5 wt%)的UHMWPE-ALN具有与UHMWPE相似的材料学性能与摩擦学性 能。通过加入表面活性剂的途径可以解决药物团聚导致的UHMWPE-ALN 1.0wt%的摩擦学性能的下降。与无摩擦状态相比,摩擦过程对 UHMWPE-ALN中ALN的释放有促进作用。 【英文摘要】Ultra-high molecular weight polyethylene (UHMWPE) has been used as a major bearing materials in total joint replacement with satisfactory clinical results due to its superior wear resistance and excellent biocompatibility. However, the wear debris of UHMWPE after implantation which would result in osteolysis and aseptic loosening has become one of the main issues that determine the longevity of artificial joint replacement. In our previous studies, UHMWPE was respectively loaded with 17β-estradiol (E2) or alendronate sodium (ALN) which were anti-osteolysis drugs in clinic. Both of UHMWPE-E2 and UHMWPE-ALN possessed satisfactory physical properties. The percentage of drug released from UHMWPE-E2 and UHMWPE-ALN during 90 days was 40% and 34%, respectively. The results of cell culture indicated that the wear debris of drug-loaded UHMWPE could prevent debris-induced osteolysis. In vivo, UHMWPE serves as one part of weight-bearing surfaces in artificial joints and guarantees the joint mobility. Thus, the wear performance of drug-loaded UHMWPE plays an important role in the human comfort and longevity of artificial joints. Furthermore, the drug release of UHMWPE-ALN in vivo would probably be affected by the stress on weight bearing joints, the relative motion-induced friction, and the wear mechanism of materials as well as the lubricants existing in peri-implants. Therefore, it is necessary to investigate the effect of loaded drug on the wear performance of UHMWPE-ALN, and the influence of friction and wear of UHMWPE-ALN on drug release rate needs to be further studied.ALN was loaded into UHMWPE with different concentration 0.25,0.5 and 1.0 wt% (named UHMWPE-ALN 0.25 wt%,0.5wt%,1.0wt%, respectively), which were processed by the solvent evaporation and hot-pressing. The Fourier Transform Infrared Spectroscopy (FTIR) and thermal analysis (TA) were carried out to investigate the thermalstability of ALN. The functional groups, crystal structure, crystallinity, contact angle, mechanical properties of UHMWPE-ALN were investigated after hot-pressing. Ball-slide-on-disc wear testing was carried out using 25 vol% calf serum or deionized water as lubricants and zirconia (ZrO2) ball as friction pair. The frictional coefficient, volume of wear scar and morphology of wear surface of UHMWPE-ALN was compared with the UHMWPE. The Secondary Electron Microscopy (SEM), Back-Scattered Electron Microscopy (BSEM) and Energy Dispersive X-Ray (EDX) revealed the distribution of ALN in UHMWPE-ALN and the effect of agglomeration of ALN on wear performance of UHMWPE-ALN. The wear performance of UHMWPE-ALN 1.0 wt% after adding surfactant (F68) was preliminarily studied. The ALN release of UHMWPE-ALN 1.0 wt% under wear process was investigated in a reciprocating mode using deionized water as lubricant.The FTIR and TA results demonstrated that the hot-pressing would not alter the functional groups of UHMWPE-ALN. X-Ray Diffraction (XRD) revealed that UHMWPE-ALN and UHMWPE had similar crystal structure. Differential Scanning Calorimetry (DSC) and contact angle testing showed that the crystallinity of UHMWPE-ALN decreased and tended to be hydrophile with the increasing concentration of ALN. UHMWPE-ALN and UHMWPE possessed similar surface hardness. The results of tensile test, small punch test and nano-scratch test indicated that the mechanical properties and scratch-resistance of UHMWPE-ALN 0.25 and 0.5wt% were similar with UHMWPE whereas those of UHMWPE-ALN 1.0 wt% decreased significantly.The friction coefficient, volume of wear scar and morphology of UHMWPE-ALN 0.25 and 0.5wt% were similar with those of UHMWPE under identical lubricant and normal load. Whereas, the friction coefficient and volume of wear scar of UHMWPE-ALN 1.0 wt% increased significantly. A lot of non-oriented loose fibrils accumulated on the worn surfaces of UHMWPE-ALN 1.0 wt% while some sheets-like peelings appeared on UHMWPE. BSE-SEM revealed that the agglomeration of ALN could probably caused the different morphology and the decrease in wear resistance of UHMWPE-ALN 1.0 wt%. By adding surfactant (F68), the friction coefficient, volume of wear scar and morphology of UHMWPE-ALN 1.0 wt% showed no significant difference as compared with UHMWPE.The ALN release during wear testing results showed that the mass of ALN released into lubricant gradually increased with the time and the concentration varied between 0.79 and 1.16 mol/L prior to 200h; and the ALN release rate increased significantly and the concentration was between 3.66 to 2.33 mol/L in the followed 200h to 310h. The ALN concentration of the control group which was soaked (without wear process) under the same lubricant was undetectable. The drug release mechanism should be further studied.The present study demonstrated that UHMWPE-ALN 0.25 and 0.5 wt% possessed similar material properties and wear performance as compared with the UHMWPE. The agglomeration of ALN caused the decrease of wear resistance of UHMWPE-ALN 1.0 wt% but it could be compensated with adding the surfactant (F68). The wear condition promoted the ALN release of UHMWPE-ALN 1.0 wt%. 【关键词】阿仑膦酸钠 超高分子量聚乙烯 骨溶解 摩擦磨损 磨 损颗粒 药物释放 【备注】索购全文在线加好友:1.3.9.9.3.8848 同时提供论文写作一对一指导和论文发表委托服务 【英文关键词】Alendronate sodium (ALN) Ultra-high molecular weight polyethylene (UHMWPE) Osteolysis Friction and wear Wear particles Drug release 【目录】载阿仑膦酸钠超高分子量聚乙烯球盘摩擦学性能研究 摘要 6-8 Abstract 8-10 第1章 绪论 13-25 1.1 人工髋关节置换术概述 13-17 1.1.1 人工 髋关节置换术的发展与现状 13-14 1.1.2 人工髋关节的组成 与材料 14-15 1.1.3 典型的髋关节摩擦配副 15-17 1.2 人工髋关节的磨损和骨溶解 17-18 1.3 骨溶解的防治 18-19 1.4 超高分子量聚乙烯(UHMWPE) 19-24 1.4.1 UHMWPE简介 19-21 1.4.2 UHMWPE摩擦学性能的摩擦试验研究 21-24 1.5 本课题研究目的以及主要 内容 财务内部控制制度的内容财务内部控制制度的内容人员招聘与配置的内容项目成本控制的内容消防安全演练内容 24-25 1.5.1 研究目的 24 1.5.2 研究内容 24-25 第2章 UHMWPE-ALN的制备与表征 25-45 2.1 引言 25 2.2 试验 材料与方法 25-30 2.2.1 UHMWPE-ALN的制备 25-27 2.2.2 ALN热稳定性测试 27 2.2.3 UHMWPE-ALN的材料学性能表征 27-28 2.2.4 UHMWPE-ALN的力学性能表征 28-30 2.3 试验结果与讨论 30-44 2.3.1 ALN的热稳定 性 30-32 2.3.2 UHMWPE-ALN的热压工艺 32-33 2.3.3 UHMWPE-ALN的材料学性能 33-37 2.3.4 UHMWPE-ALN的力学性 能 37-44 2.4 小结 44-45 第3章 UHMWEP-ALN的摩擦学 性能 45-68 3.1 引言 45 3.2 摩擦试验设备与方法 45-49 3.2.1 试验设备 45 3.2.2 试验材料 45-46 3.2.3 试验工况与方法 46-49 3.3 UHMWPE-ALN摩擦学特性 49-62 3.3.1 载药浓度对摩擦学性能的影响 49-58 3.3.2 法向载荷对摩擦学性能的影响 58-59 3.3.3 润滑液对摩擦学性能的影响 59-62 3.4 改善药物分散性后的UHMWPE-ALN 1.0 WT%摩擦学性能 62-67 3.4.1 加表面活性剂的UHMWPE-ALN 1.0 wt%(F68-ALN 1.0 wt%)的制备 63 3.4.2 25vol%胎牛血清润滑下摩擦学特性 63-65 3.4.3 水润滑下摩擦学特性 65-67 3.5 小结 67-68 第4章 摩擦对UHMWPE-ALN药物释放的影响 68-75 4.1 引言 68 4.2 摩擦控释试验参数与方法 68-70 4.2.1 摩擦试验工况 68 4.2.2 取液方式 68-69 4.2.3 液体中ALN的浓度测定 69-70 4.3 结果与讨论 70-74 4.3.1 标准 excel标准偏差excel标准偏差函数exl标准差函数国标检验抽样标准表免费下载红头文件格式标准下载 曲线 70-71 4.3.2 润滑液中ALN的显色反应 71-72 4.3.3 润滑液中ALN的浓度 72-74 4.4 小结 74-75 结论 75-76 展望 76-77 致谢 77 攻读硕士学位期间发表的论文 77-78 参考文献 78-84