免疫试题03七的两套试题打印版

啄 木 论 坛 [ www.zhuomu.cn ] V I P 资 料 免 疫 学 分 册 【试题】 The final examination of medical immunology for seven year students of Grade 2003(2005/12/6) Paper A I. Explain the following items (2 points for each, 18 points) 1. Lymphocyte recirculation 2. CDR 3. cytokine 4. MHC restriction 5. PRR 6. Immune tolerance 7. ITAM 8.APC 9. TI-Ag II. Choose all correct answers (1 point for each,15 points) 1. Which of the following statement(s) about the spleen is(are) true: A. It filters antigens out of the blood. B. Lymphatic vessels draining the tissue spaces enter the spleen C. It contains germinal centers D. It functions to remove old and defective red blood cells 2.Which kinds of Ig expressed mainly on the surface of mature B cells? A. mIgG B. mIg A C. mIgD D.mIgM 3.Which can function as C3 convertase in activation of complement system? A.C4b2b B.C4b2b3b C.C3bBb D.C3bnBb 4.About the characteristics of cytokines, which of the following(s) is (are) correct? A. One cytokine can act on different cells and play multiple biological effects B. The cytokine produced by one cell only acts on neighbor cells C. Combined with corresponding receptor to play role D. One cytokine can inhibit or enhance effects of other cytokine 5.Which of the following molecules can stimulate T cells activation? A. LPS B. PWM C. Con A D. PHA 6.What are the functions of B cells? A. produce Ab B. secrete cytokines C. kill target cells directly D. present Ag 7.Which of the cytokines are secreted by Th1 cells? A. IL-4 B. IL-2 C. IL-10 D. IFN-γ 8.Which of the diseases belong to type Ⅲ hypersensitivity? A. Transfusion reaction B. Arthus reaction C. Contact dermatitis D. Rheumatoid arthritis 9.Which of the following is(are) correct about the characteristics of memory cells? A. Can come from either B cell or T cell B. Can respond to antigen at low consentrate effectivelly C. Only exist in HI D. React to a second invasion of an antigen more rapidly and stronger 10.What abilities do αβ+T cells gain after maturation? A. self-reactive B. self-tolerance C. express functional TCR D. self MHC restriction 11.Which of the followings are correct statements about dendritic cells? A. Can stimulate na?ve T cell proliferation B. Express high level of MHC class II molecules C. Express high level of MHC class I molecules D. Mature dendritic cells with strong function of antigen-capture 12.About AICD, which statements are correct? A. Can be mediated by FasL-Fas interaction B. Is a kind of cell death of activated T cell C. Play important roles in immunological regulation D. IL-4 can promote AICD 13. The processes of antigen-activated B cells differentiation in germinal center include: A. Ig Class switching B. Somatic hypermutation and Ig affinity maturation C. Differentiate into Bm D. Apoptosis 14.Which of the following factors affect the antigen immunogenecity? A. molecule weight B. foreigness C. conformation D. chemical composition 15. About somatic hypermutation, which statements are correct? A. Occurs during B cells maturation B. Result in affinity maturation of antibodies C. Mainly occurs in CDR region D. Can occur during T cells maturation Ⅲ．Answer the following questions briefly (5 point for each, 30 points) 1. How can T helper cells play important roles in an antibody mediated response. 2.What cells express the HLA II molecules? What are the functions of HLA II molecules on these cells? 3.If we want to label the antibody with enzyme for ELISA experiment, what part of antibody does enzyme should bind to? Why? 4.How do effector specific CTLs kill tumor cells? What are the characteristics for this process? 5. How are endogenous antigens recognized by CD8+ T cells? 6. What is Neonate hemolysis? Please describe its mechanism. IV. Answer following question (12 points) 1. How the innate and adaptive immunity act in cooperative and inter-dependent ways to protect the host.? 【Answer】 I. Explain the following items (2 points for each, 18 points) 1.Lymphocyte recirculation: is the process by which lymphocytes continuously move between sites throughout the body(1) through blood and lymphatic vessels, and it is critical for the initiation and effector phases of immune responses(1). 2. CDR: Complementarity-determining region (0.5) the three HVR of L chain and H chain are brought together to form antigen-binding surface, so HVR is also called CDR(1) 3. cytokine: A group of(0.5) low molecule weight proteins(0.5) which are secreted by activated immunocytes or some matrix cells(0.5) and take effects by binding corresponding receptor on cells(0.5). 4.MHC restriction: In interaction of T cell and antigen-presenting cell(APC) or target cell(0.5) ,T cell not only recognizes specific antigen peptide, but also recognizes polymophic residue of MHC molecules(0.5). 5. Pattern recognition receptor(PRR): The receptor on macrophage(0.5) which can recognize and bind specific molecular structure on some pathogens , injured or apoptotic cells(0.5). 6. Immune tolerance a specific nonreactivity 1.0 towards a given antigen 0.5 in other circumstance is able to induce cell-mediated or humoral immunity 0.5 7. ITAM immunoreceptor tyrosine-based activation motif 0.5 transduct activation signal 1.0 composed of two tyrosine residues separated by around 18 amino acid-----when antigen binds, the tyrosine become phosphorylated by the receptor associated tyrosine kinases to transduct activatory signal 0.5 8.APC cells that can capture（0.5）, process antigen （0.5）, and present the antigenic peptide/MHC class I or II molecules（0.5） to T cells （0.5） are called antigen-presenting cells. They are also called A cell (Accessory cell). 9. TI-Ag (thymus independent Ag) : Ag which can stimulate B cells to produce Ab(0.5) without the help of T cell and MФ(0.5). II. Choose all correct answers (1 point for each, 15 points) No. 1 2 3 4 5 6 7 8 Answer ACD CD AC ACD BCD ABD BD BD No. 9 10 11 12 13 14 15 Answer ABD BCD ABC ABC ABCD ABCD ABC Ⅲ．Answer the following questions briefly (5 point for each, 30 points) 1. How can T helper cells play important roles in an antibody mediated response. (1) Express CD40L(0.5),combine with CD40 expressing on B cell(0.5),provide co-stimulation signal during the activation of B cell(1). (2) Secrete IL-4,5,6(0.5) etc. to promote proliferation and differentiation of B cell(1) (3) Secrete cytokines to promote Ig isotype switching(1.5). 2.What cells express the HLA II molecules? What are the functions of HLA II molecules on these cells? cells express the HLA II molecules (2) professional APC(macrophage, dentritic cell and B cell), activated T cell, thymus epithelial cell et al. the functions of HLA II molecules (1 point each) ( Participate in controlling Th cell- APC recognition in immunoreaction ( Participate in processing and presenting of antigen ( Participate in inducing the differentiation and development of T cell in thymus 3.If we want to label the antibody with enzyme for ELISA experiment, what part of antibody does enzyme should bind to? Why? Fc fragment(2),because: Fab binds to Ag specifically(1) Fc has no effect on Ag binding, so Fc can be labeled to detect Ag-Ab complex without effection on its Ag binding activity(2) 4.How do effector specific CTLs kill tumor cells? What are the characteristics for this process? • Specific recognition and binding of target cell by CTL • Lethal hit to target cell • Perforin 1 • Granzyme 1 • Fas/FasL pathway—apoptosis 0.5 • Specific killing 1 • classⅠMHC molecule restriction 1 • Continuous killing of target cells in short time, and no injury of CTL 0.5 5. How are endogenous antigens recognized by CD8+ T cells? ( By class I MHC pathway 1 ( Processing of endogenous Ag：proteosome -LMP2, LMP7，antigen peptides(8-13aa) 1 ( Transporting of antigen peptide into ER：TAP(transporter associated with antigen processing) 1 ( Peptide loading of MHC I molecules：ER，antigen peptide—class Ⅰ MHC complexes 1 ( Presenting to CD8+T cells：TCR-peptide, CD8-α3 1 6. What is Neonate hemolysis? Please describe its mechanism. Definition: Maternal IgG specific for the fetal blood groups crosses the placenta and destroys the fetal red blood cells 1 Mechanism: Mainly induced by Rh antigen. It is Type II hypersensitivity 0.5 Rh antigens on the surface of fetal red blood cells induce IgG antibody production within mother body---the first pregnancy. 1 IgG crosses the placenta and bind with Rh antigen to form Ag-Ab complex---the second pregnancy. 1 Cell lysis: Ag-Ab complex activates complement by classic pathway. MAC 0.5 ADCC 0.5 Oposinization by C and Ab promotes macrophages function. 0.5 IV. Answer following question (12 points) 1. How do the innate and adaptive immunity act in cooperative and inter-dependent ways to protect the host? Innate immunity → adaptive immunity( 8 points) (1)macrophage and DC participate in antigenic presentation and initiate adaptive immunity 3 points (2)macrophage,NK,Complement etc. participate in effector phage of adaptive immunity 3 points (3)macrophage and NK secrete cytokines regulate types of adaptive imunity 2 points Adaptive immunity→Innate immunity(4 points) (1) Ab-Ag complex situmulate activation of complement by classical pathway 1 points (2) Activated T cells secrete cytokines to participate in innate immunity 1 points (3) Activated T cells secrete cytokines to activate NK cell 1 points (4) Activated T cells secrete cytokines to activate macrophage 1 points 【试题】 The final examination of medical immunology for seven year students of Grade 2003(2005/12/6) Paper B I. Explain the following items (2 points for each, 18 points) 1. McAb 2. MAC 3. Hapten 4. TCR complex 5. Hypersensitivity 6. Clonal anergy 7. Heterophile Ag 8.ITAM 9.PRR II. Choose all correct answers (1 point for each,15 points) 1. Which of the following statement(s) about the spleen is(are) true: A. It filters antigens out of the blood. B. Lymphatic vessels draining the tissue spaces enter the spleen C. It contains germinal centers D. It functions to remove old and defective red blood cells 2.Which kinds of Ig expressed mainly on the surface of mature B cells? A. mIgG B. mIg A C. mIgD D.mIgM 3.Which can function as C3 convertase in activation of complement system? A.C4b2b B.C4b2b3b C.C3bBb D.C3bnBb 4.About the characteristics of cytokines, which of the following(s) is (are) correct? A. One cytokine can act on different cells and play multiple biological effects B. The cytokine produced by one cell only acts on neighbor cells C. Combined with corresponding receptor to play role D. One cytokine can inhibit or enhance effects of other cytokine 5.Which of the following molecules can stimulate T cells activation? A. LPS B. PWM C. Con A D. PHA 6.What are the functions of B cells? A. produce Ab B. secrete cytokines C. kill target cells directly D. present Ag 7.Which of the cytokines are secreted by Th1 cells? A. IL-4 B. IL-2 C. IL-10 D. IFN-γ 8.Which of the diseases belong to type Ⅲ hypersensitivity? A. Transfusion reaction B. Arthus reaction C. Contact dermatitis D. Rheumatoid arthritis 9.Which of the following is(are) correct about the characteristics of memory cells? A. Can come from either B cell or T cell B. Can respond to antigen at low consentrate effectivelly C. Only exist in HI D. React to a second invasion of an antigen more rapidly and stronger 10.What abilities do αβ+T cells gain after maturation? A. self-reactive B. self-tolerance C. express functional TCR D. self MHC restriction 11.Which of the followings are correct statements about dendritic cells? A. Can stimulate naïve T cell proliferation B. Express high level of MHC class II molecules C. Express high level of MHC class I molecules D. Mature dendritic cells with strong function of antigen-capture 12.About AICD, which statements are correct? A. Can be mediated by FasL-Fas interaction B. Is a kind of cell death of activated T cell C. Play important roles in immunological regulation D. IL-4 can promote AICD 13. The processes of antigen-activated B cells differentiation in germinal center include: A. Ig Class switching B. Somatic hypermutation and Ig affinity maturation C. Differentiate into Bm D. Apoptosis 14.Which of the following factors affect the antigen immunogenecity? A. molecule weight B. foreigness C. conformation D. chemical composition 15. About somatic hypermutation, which statements are correct? A. Occurs during B cells maturation B. Result in affinity maturation of antibodies C. Mainly occurs in CDR region D. Can occur during T cells maturation Ⅲ．Answer the following questions briefly (5 point for each, 30 points) 1.Why do NK cells only kill tumor or virus-infected cells but not normal cells? 2.Describe functions of three kinds of adhesion molecules which you know. 3. How to use immunologic tolerance in clinical medicine? Please give more than two examples. 4. Which type of hypersensitivity does allergy asthma belong to? How does it take place? 5.How was complement activated in early phage of bacterial infection? 6. Where are the CDR regions located on the antibody molecule and what are their fuctions? IV. Answer following question (12 points) 1. When microbes invade an individual from skin,how can this individual eliminate them? Please describe the overall process. 【Answer】 ⅠExplain the following items （2 points for each,18 points） 1. McAb Prepared by hybridomas technique (1 point) Immunized spleen cells(B) hybride with myeloma cells hybridoma (1 point) 2. MAC C5b6789 complex is known as MAC which leads to formation of a hole in the membrane.(1 point) MAC mediates lysis of target cell.(1 point) 3. Hapten Substances which can combine with Ab, but cannot induce immune response independently.(1 point) In another word, hapten only possess immunoreactivity.(1 point) 4.TCR complex a group of membrane molecules on T cells that can specially bind to the antigen and pass an activation signal into the cells (1 point) consisting of TCR, CD3 and zz (1 point) 5.Hypersensitivity a harmful immune response that is heightened reactivity to an antigen is called hypersensitivity, (1 point) which causes tissue injury or functional disorder.(1 point) 6.Clonal anergy functional inactivation without cell death(1 point) : lack co-stimulatory signal(1 point). 7.Heterophile Ag (forssman Ag):common Ags are shared by different species -no specificity of species (1 point) - Significance : immunopathology ,Diagnosis (1 point) 8.ITAM immune receptor tyrosine-based activation motif (0.5 point)-----transduce activation signals from TCR(1 point) composed of tyrosine residues separated by around 18 amino acid-----when antigen binds,the tyrosine become phosphorylated by the receptor associated tyrosne kinas to transduct activtory signal(0.5 point) 9.PRR The receptor on macrophage (1 point)which can recognize and bind specific molecular structure on some pathogens,injured or apoptotic cells. (1 point) II. Choose all correct answers (1 point for each, 15 points) No. 1 2 3 4 5 6 7 8 Answer ACD CD AC ACD BCD ABD BD BD No. 9 10 11 12 13 14 15 Answer ABD BCD ABC ABC ABCD ABCD ABC Ⅲ。Answer the following questions briefly(5 point for each, 30points) 1. There are two different receptors expressed on NK cells: KAR which can recognized glucide ligand distributing on tumor cells and virus-infected cells and transduct activtory signals by ITAM structure and KIR which can recognize MHC-Ⅰexpressed on self-organic cells and transduct inhibitory signals by ITAM structure. 2. 1). Ig superfamily(IgSF) Act as co-receptor and co-stimulatory signal during the recognition and activation of immune cells. 2).Integrin family Participate in the adhesion of leukocyte and vascular endothelial cell during inflammation. 3) Participate in lymphocyte homing. Mature lymphocyte migrate to peripheral lymphoid organs Recirculation of lymphocytes Lymphocyte migrate to the sites of inflammation 3. 1). Induction of immunologic tolerance to antigen in fetal period and neonate period Prevent the rejection of organ allografts and xenografts Treat autoimmune diseases Treat allergic diseases 2). To terminate immunologic tolerance To treat tumor: enhance first signal or second signal To treat infection diseases 4. Type I； 1） Allergen-stimulated period Antigen binding to IgE cross-links FcεRⅠ molecules on the mast cells induces the release of mediators (degranulation)： 2）Sensitization against allergens 3）The activators of mast cells Mast cell activation products： histamine leukotrienes------Preformed mediators 5. 1) Alternative pathway: Stabilization of C3 convertase ------- Certain bacteria or their products (lipid polysaccharides, etc.), provide a protected (activator) surface for C3b and C3bBb.Morover, binding of another protein,factor P, further stabilizes this complex Generation of C5 convertase -------- Stabilized C3 convertase cleaves more C3 and produces C3bnBb complex , the C5 convertase which cleaves C5 into C5a and C5b. 2 )MBL pathway : (1)binding stage Mannan-binding lectin (MBL) binds mannose of bacteria and interacts with serine protease resulting in MBL-associated serine protease (MASP). MASP is analogous to C1 and leads to cleaving of C4 and C2. (C3 convertase) (2) Activation stage 6. There are three highly diversity stretches within the V egion which called CDR Ag-binding sites Idiotype of Ig Ⅳ Answer following question (12 points) 1. innate immune 1) Activation of complement system : Cell lysis, Opsonization ,ADCC, elimination of immune complex 2) Non-specific immune cells: macrophage cells(eosinophil ,Mφ ect)，NK cells(ADCC) or by serecting many cell factors. 2. adptive immune 1) T immune response :need APC to capture,process antigen,and present the antigenic peptide/MHC classⅠorⅡmolecules to T cells.(CD4+T and CD8+T cells) 2) B immune response: by serecting Ig阿 PAGE 1