兔富血小板血浆制备及其活性分析

中国组织工程研究   2013, Vol. 17   Issue (8): 1411-1417     DOI: 10.3969/j.issn.2095-4344.2013.08.014 生物材料基础实验 basic experiments of biomaterials 兔富血小板血浆制备及其活性 分析 定性数据统计分析pdf销售业绩分析模板建筑结构震害分析销售进度分析表京东商城竞争战略分析 Preparation and activity of rabbit platelet-rich plasma 全文: PDF (530 KB)  摘要 背景：富血小板血浆是目前已知富含多种生长因子并能将其释放的自体提取物，并已应用于骨、软骨组织工程再生的研究。目的：通过比较不同方法制备兔富血小板血浆中血小板浓度，并测定其血小板源性生长因子、转化生因子β1水平，探讨富血小板血浆制备方法及影响因素。方法：采用Petrungaro法、Landesberg法、Aghaloo法制备新西兰大耳白兔富血小板血浆。检测3组富血小板血浆中血小板计数，以及3组富血小板血浆活化前后及正常血浆、贫血小板血浆中血小板源性生长因子、转化生因子β1水平。结果与结论：3种方法制备的富血小板血浆中血小板计数、血小板回收率、血小板富集系数差异有非常显著性意义(P < 0.001)，Landesberg法和Aghaloo法均可制备有效浓度的富血小板血浆，且Aghaloo法制备血小板浓度及活性高于Landesberg法(P < 0.05)。活化前3组富血小板血浆中血小板源性生长因子、转化生因子β1水平与正常血浆组、贫血小板血浆组比较差异无显著性意义。活化后，Landesberg法和Aghaloo法制备的血小板血浆中血小板源性生长因子、转化生因子β1水平明显高于活化前(P < 0.001)，且Aghaloo法最高(P < 0.05)。富血小板血浆中血小板计数与血小板源性生长因子水平(r=0.872，P < 0.001)，转化生因子β1水平(r=0.917，P < 0.001)呈正相关。 关键词：生物材料；生物材料基础实验；富血小板血浆；血浆；血小板；血小板源性生长因子；转化生长因子；血小板活化；软骨组织；活化；组织工程；组织再生；其他基金；生物材料图片文章； Abstract： BACKGROUND:Platelet-rich plasma is an autogeneic extract containing and releasing a variety of growth factors, which has been used in the research of bone and cartilage tissue engineering and regeneration.OBJECTIVE:To investigate the preparation methods of platelet-rich plasma and its influencing factors by comparing the platelet concentration in the platelet-rich plasma prepared by different methods in rabbits and detecting the levels of platelet-derived growth factor and transforming growth factor β1.METHODS:Platelet-rich plasma was prepared by the methods of Petrungaro, Landesberg and Aghaloo. Platelet amount was counted in the platelet-rich plasma prepared using three methods as well as platelet-derived growth factor and transforming growth factor β1 levels.RESULTS AND CONCLUSION:There were significant differences in the platelet count, platelet recovery rate and platelet enrichment coefficient in the platelet-rich plasma prepared by these three methods (P < 0.001). The effective concentration of platelet-rich plasma could be prepared by the methods of Landesberg and Aghaloo, and the platelet concentration and activity prepared by Aghaloo method was higher than that prepared by Landesberg method with a statistical difference (P < 0.05). The levels of platelet-derived growth factor and transforming growth factor β1 in the inactivated platelet-rich plasma were not statistically different from those in the normal plasma and platelet-poor plasma. The levels of platelet-derived growth factor and transforming growth factor β1 in the activated platelet-rich plasma prepared by Landesberg and Aghaloo methods were significantly higher than before activation (P < 0.001), especially in the activated platelet-rich plasma prepared by Aghaloo method (P < 0.05). The platelet count in the platelet-rich plasma was positively correlated with the levels of platelet-derived growth factor (r=0.872, P < 0.001) and transforming growth factor β1 (r=0.917, P < 0.001). Key words：biomaterials;basic experiments of biomaterials;platelet-rich plasma;plasma;platelet;platelet-derived growth factor;transforming growth factor;platelet activation;cartilage;activation;tissue engineering;tissue regeneration;other grants-supported paper;biomaterial photographs-containing paper; 收稿日期:2012-06-08   研究亮点: 1  实验采用3种不同的离心方法制备提取富血小板血浆，并对其相关参数及活性进行比较。 2  富血小板血浆可提供多种自体促生长因子，促进骨髓间充质干细胞、软骨细胞增殖分化。 3   实验中发现：①Petrungaro法首次较大离心力造成红细胞、血小板的沉降，因此在白膜层下的红细胞中夹杂着大量的血小板，使得首次离心后上层血浆中只含有很少的血小板。Landesberg法、Aghaloo法首次离心力小，可使沉降速率大的红细胞首先沉降于管底，而对血小板的影响相对较小，故大部分血小板仍位于上清血浆中。Landesberg法第2次离心力不变，此离心力不能使上层血浆中全部的血小板沉降于管底，所以血小板回收率低于Aghaloo法。 4  富血小板血浆活化后还会释放其他多种生长因子，包括碱性成纤维细胞生长因子、胰岛素样生长因子、血管内皮生长因子、内皮细胞生长因子等其它活性因子，本实验因资金的问 题 快递公司问题件快递公司问题件货款处理关于圆的周长面积重点题型关于解方程组的题及答案关于南海问题 仅对血小板源性生长因子，转化生长因子进行了测定，故不能全面了解富血小板血浆活化后其他因子含量的变化。