大肠杆菌基因型大全

2015/5/26E. coli genotypes ­ OpenWetWareE.coligenotypesFromOpenWetWareContents1Nomenclature&Abbreviations2MethylationIssuesinE.coli3Commonlyusedstrains3.1AG13.2AB11573.3B21553.4BL213.5BL21(AI)3.6BL21(DE3)3.7BL21(DE3)pLysS3.8BNN933.9BNN973.10BW26434,CGSCStrain#76583.11C6003.12C600hflA150(Y1073,BNN102)3.13CSH503.14D12103.15DB3.13.16DH13.17DH5α3.18DH5αTurbo(NEB)3.19DH10B(Invitrogen)3.20DH12S(Invitrogen)3.21DM1(Invitrogen)3.22E.cloni(r)5alpha(Lucigen)3.23E.cloni(r)10G(Lucigen)3.24E.cloni(r)10GF'(Lucigen)3.25E.coliK12ER2738(NEB)3.26ER2566(NEB)3.27ER2267(NEB)3.28HB1013.29HMS174(DE3)3.30High-Control(tm)BL21(DE3)(Lucigen)3.31High-Control(tm)10G(Lucigen)3.32IJ11263.33IJ11273.34JM833.35JM1013.36JM1033.37JM1053.38JM1063.39JM1073.40JM1083.41JM1093.42JM109(DE3)3.43JM1103.44JM2.3003.45LE3923.46M15(Qiagen)3.47Mach13.48MC10613.49MC41003.50MFDpir3.51MG16553.52OmniMAX23.53OverExpress(tm)C41(DE3)(Lucigen)3.54OverExpress(tm)C41(DE3)pLysS(Lucigen)3.55OverExpress(tm)C43(DE3)(Lucigen)3.56OverExpress(tm)C43(DE3)pLysS(Lucigen)3.57Rosetta(DE3)pLysS3.58Rosetta-gami(DE3)pLysS3.59RR13.60RV3083.61SOLR(Stratagene)3.62SS320(Lucigen)3.63STBL2(Invitrogen)3.64STBL3(Invitrogen)3.65STBL43.66SURE(Stratagene)3.67SURE2(Stratagene)3.68TG1(Lucigen)3.69TOP10(Invitrogen)3.70Top10F'(Invitrogen)3.71W31103.72W3110(λ857S7)3.73WM3064http://openwetware.org/wiki/E._coli_genotypes1/142015/5/26E. coli genotypes ­ OpenWetWare3.74XL1-Blue(Stratagene)3.75XL1-BlueMRF'(Stratagene)3.76XL2-Blue(Stratagene)3.77XL2-BlueMRF'(Stratagene)3.78XL1-Red(Stratagene)3.79XL10-Gold(Stratagene)3.80XL10-GoldKanR(Stratagene)4Othergenotypeinformationsources5ReferencesNomenclature&AbbreviationsAlistedgenenamemeansthatgenecarriesalossoffunctionmutation,aΔprecedingagenenamemeansthegeneisdeleted.Ifageneisnotlisted,itisnotknowntobemutated.ProphagespresentinwtK-12strains(F,λ,e14,rac)arelistedonlyifabsent.E.coliBstrainsarenaturallylon-anddcm-.F-=DoesnotcarrytheFplasmidF+=CarriestheFplasmid.ThecellisabletomatewithF-throughconjugation.F'[]=CarriesanFplasmidthathashostchromosomalgenesonitfromapreviousrecombinationevent.ThiscellcanalsomatewithF-throughconjugation.ChromosomalgenescarriedintheFplasmidarelistedinbrackets.+/-rB/K=The(B/K)definesthestrainlineage.The+/-indicateswhetherthestrainhasorhasn'tgottherestrictionsystem.+/-mB/K=The(B/K)definesthestrainlineage.The+/-indicateswhetherthestrainhasorhasn'tgotthemodification(methylation)system.hsdS=Bothrestrictionandmethylationofcertainsequencesisdeletedfromthestrain.IfyoutransformDNAfromsuchastrainintoawildtypestrain,itwillbedegraded.hsdR=ForefficienttransformationofclonedunmethylatedDNAfromPCRamplificationsINV()=chromosomalinversionbetweenlocationsindicatedahpC=mutationtoalkylhydroperoxidereductaseconferringdisulfidereductaseactivityara-14=cannotmetabolizearabinosearaD=mutationinL-ribulose-phosphate4-epimeraseblocksarabinosemetabolismcycA=mutationinalaninetransporter;cannotusealanineasacarbonsourcedapD=mutationinsuccinyldiaminopimelateaminotransferaseleadstosuccinateor(lysine+methionine)requirementΔ()=chromosomaldeletionofgenesbetweenthelistedgenes(mayincludeunlistedgenes!)dam=adeninemethylationatGATCsequencesexist;highrecombinationefficiency;DNArepairturnedondcm=cytosinemethylationatsecondCofCCWGGsitesexist.dam&dcmarethedefaultpropertiesandalwayselided,whiledam-ordcm-shouldbedeclareexplicitlyDE3(http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/Transformation/expression-competent-cells.html)=LysogenthatencodesT7RNApolymerase.UsedtoinduceexpressioninT7-drivenexpressionsystemsdeoR=regulatorygenethatallowsconstitutiveexpressionofdeoxyribosesynthesisgenes;permitsuptakeoflargeplasmids.SeeHanahanD,USPatent4,851,348(http://patft.uspto.gov/netacgi/nph-Parser?u=/netahtml/srchnum.htm&Sect1=PTO1&Sect2=HITOFF&p=1&r=1&l=50&f=G&d=PALL&s1=4851348.WKU.&OS=PN/4851348&RS=PN/4851348).***Thishasbeencalledintoquestion,astheDH10BgenomesequencerevealedthatitisdeoR+.SeeDurfee08,PMID18245285.dnaJ=oneofthechaparoninsinactivated;stabilizessomemutantproteinsdut1=dUTPaseactivityabolished,leadingtoincreaseddUTPconcentrations,allowinguracilinsteadofthymineincorporationinDNA.StableUincorporationrequiresunggenemutationaswell.endA1=ForcleanerpreparationsofDNAandbetterresultsindownstreamapplicationsduetotheeliminationofnon-specificdigestionbyEndonucleaseI(e14)=excisableprophagelikeelementcontainingmcrAgene;presentinK-12butmissinginmanyotherstrainsgalE=mutationsareassociatedwithhighcompetence,increasedresistancetophageP1infection,and2-deoxygalactoseresistance.galEmutationsblocktheproductionofUDP-galactose,resultingintruncationofLPSglycanstotheminimal,"innercore".TheexceptionalcompetenceofDH10B/TOP10isthoughttobearesultofareducedinterferencefromLPSinthebindingand/oruptakeoftransformingDNA.galE15isapointmutationresultinginaSer123->Pheconversionneartheenzyme'sactivesite.SeevanDie,etal.PMID6373734,Hanahan,etal.PMID1943786,andEcoSalISBN1555811647.--Dcekiert16:56,23January2008(CST)galk=mutantscannotmetabolizegalactoseandareresistantto2-deoxygalactose.galK16isanIS2insertion~170bpdownstreamofthegalKstartcodon.SeeEcoSalISBN1555811647.--Dcekiert16:56,23January2008(CST)galU=mutantscannotmetabolizegalactosegor=mutationinglutathionereductase;enhancesdisulphidebondformationglnV=suppressionofamber(UAG)stopcodonsbyinsertionofglutamine;requiredforsomephagegrowthgyrA96=mutationinDNAgyrase;conveysnalidixicacidresistancegyrA462=mutationinDNAgyrase;conveysresistancetoccdBcolicingeneproducthflA150=proteasemutationstabilizingphagecIIprotein;highfrequencyoflysogenizationbyλΔ(lac)X74=DeletionoftheentirelacoperonaswellassomeflankingDNA(completedeletionisΔcod-mhpF;seeMol.Micro.,6:1335,andJ.Bact.,179:2573)lacIqorlacIQ=overproductionofthelacrepressorprotein;-35siteinpromoterupstreamoflacIismutatedfromGCGCAAtoGTGCAAlacIQ1=overproductionofthelacrepressorprotein;containsa15bpdeletiontocreateoptimal-35siteinpromoterupstreamoflacIlacY=deficientinlactosetransport;deletionoflactosepermease(Mprotein)lacZΔM15=partialdeletionofthelacZgenethatallowsαcomplementationoftheβ-galactosidasegene;requiredforblue/whiteselectiononXGalplates.DeletestheaminoportionoflacZ(aa11-41).LAM-orλ-=lambdalysogendeletion;approximatemaplocation:17.40;informationfromCGSC(http://cgsc.biology.yale.edu/Mutation.php?ID=4499)*---Karmella13:02,21October2012(EDT):LamR=mutationinmalT1conferringlambdaresistance;synonymmalT1(LamR)[1](http://cgsc.biology.yale.edu/Mutation.php?ID=4749)*---Karmella13:35,21October2012(EDT):leuB=requiresleucineΔlon=deletionofthelonproteasemalA=cannotmetabolizemaltosemcrA=MutationeliminatingrestrictionofDNAmethylatedatthesequenceCmCGG(possiblymCG).Carriedonthee14prophage(q.v.)mcrB=MutationeliminatingrestrictionofDNAmethylatedatthesequenceRmChttp://openwetware.org/wiki/E._coli_genotypes2/142015/5/26E. coli genotypes ­ OpenWetWaremetB=requiresmethioninemetC=requiresmethioninemrr=MutationeliminatingrestrictionofDNAmethylatedatthesequenceCmAGorGmACmtlA=cannotmetabilizemannitol(Mu)=Muprophagepresent.Muδmeansthephageisdefective.mutS-mutationinhibitsDNArepairofmismatchesinunmethylatednewlysynthesizedstrandsnupG=sameasdeoRompT=mutationinoutermembraneproteinproteaseVII,reducingproteolysisofexpressedproteins(P1)=CellcarriesaP1prophage.CellsexpresstheP1restrictionsystem.(P2)=CellcarriesaP2prophage.AllowsselectionagainstRed+Gam+λ(φ80)=Cellcarriesthelambdoidprophageφ80.AdefectiveversionofthisphagecarryinglacZM15deletion(aswellaswild-typelacI,lacYA,andflankingsequences)ispresentinsomestrains.Theφ80attachmentsiteisjustadjacenttotonB.pLysS=containspLysSplasmidcarryingchloramphenicolresistanceandphageT7lysozyme,effectiveatattenuatingactivityofT7RNApolymerase,forbetterinhibitionofexpressionundernon-inducedconditions.Thesequencecanbefoundhere(http://www.emdbiosciences.com/docs/NDIS/69659-000.HTML).proA/B=requiresprolinerecA1=ForreducedoccurrenceofunwantedrecombinationinclonedDNA;cellsUVsensitive,deficientinDNArepairrecA13=asforrecA1,butinsertslessstable.recBCD=ExonucleaseV;mutationinRecBorRecCreducesgeneralrecombinationbyafactorof100;impairedDNArepair;UVsensitive,easierpropagationofinvertedrepeatsrecJExonucleaseinvolvedinalternaterecombinationrelA=relaxedphenotype;permitsRNAsynthesisinabsenceofproteinsynthesisrha=blockedrhamosemetabolismrnc=encodesRnaseIII(rnc-14isacommonnullmutant)rne=encodesRnaseE(rne-3071isacommontemperaturesensitivemutant)rpsL=mutationinribosomalproteinS12conveyingstreptomycinresistance;alsocalledstrA,rpsL135(strR),strA135[2](http://cgsc.biology.yale.edu/Mutation.php?ID=5280)*---Karmella13:27,21October2012(EDT):sbcBC=ExoIactivityabolished;usuallypresentinrecBCstrains;recombinationproficient,stableinvertedrepeatssr1=cannotmetabolizesorbitolsupE=glnVsupF=tyrTthi=requiresthiaminethyA=requiresthymidineTn10=transposonnormallycarryingTetracyclineresistanceTn5=transposonnormallycarryingKanamycinresistancetonA=MutationinoutermembraneproteinconveyingresistancetophageT1andphageT5traD=Mutationeliminatingtransferfactor;preventstransferofFplasmidtrxB=mutationinthioredoxinreductase;enhancesdisulphidebondformationinthecytoplasmtsx=outermembraneproteinmutationconveyingresistancetophageT6andcolicinKtyrT=suppressionofamber(UAG)stopcodonsbyinsertionoftyrosine;neededforsomephageinfectionsuchasλgt11.ung1=allowsuraciltoexistinplasmidDNAxyl-5=blockedxylosemetabolismSmR=StreptomycinresistanceMethylationIssuesinE.coliTypeImethylationsystems:**E.coliK12restrictsDNAwhichisnotprotectedbyadeninemethylationatsitesAAC[N6]GTGCorGCAC[N6]GTT,encodedbythehsdRMSgenes(EcoKI).Deletionsinthesegenesremoveseithertherestrictionormethylationorbothofthesefunctions.**E.coliBderivativestrainscontainanhsdRMSsystem(EcoBI)restrictingandprotectingthesequenceTGA[N8]TGCTorAGCA[N8]TCA.ThemcrAgene(carriedonthee14prophage)restrictsDNAwhichismethylatedinCmCWGGormCGsequences(methylationbythedcmgeneproduct).ThemcrBCgenesrestrictRmCsequences.ThemrrgeneproductrestrictsadeninemethylatedsequencesatCAGorGACsites.E.colimethylatestheadenineinGATC(andthecorrespondingAontheoppositestrand)withthedamgeneproduct.M.EcoKIImethylatesthefirstAatthepalindromicsiteATGCAT(aswellasthecorrespondingAontheoppositestrand),see(KossykhVG(2004)J.Bact186:2061-2067PMID15028690)Notethatthisarticlehasbeenretracted;theretractionappearstocenterontextualplagarism,notexperimentalresults.ThehomologytoAvaIIIisreal.IthinkIbelieveit.tk20:28,9December2005(EST).RichRobertsreports:"Wehavetriedourselvestodetectactivitywiththisgeneproductandcannotdetectanymethyltransferaseactivity.InourcaseweusedantibodiesabletodetectN6-methyladenineorN4methylcytosineinDNA.Theoneswehaveareverysensitiveandshouldhavebeenabletodetect5-methylgroupsinthewholeE.colichromosome.Nothingwasdetectedinanover-expressingstrain."ForadditionalinformationseeE.colirestriction-modificationsystemandtheNEBtechnicalinformationonmethylation(http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/making_unmethylated_dna.asp).CommonlyusedstrainsAG1-+endA1recA1gyrA96thi-1relA1glnV44hsdR17(rKmK)AB1157thr-1,araC14,leuB6(Am),Δ(gpt-proA)62,lacY1,tsx-33,qsr'-0,glnV44(AS),galK2(Oc),LAM-,Rac-0,hisG4(Oc),rfbC1,mgl-51,rpoS396(Am),rpsL31(strR),kdgK51,xylA5,mtl-1,argE3(Oc),thi-1BachmannBJ:DerivationandgenotypesofsomemutantderivativesofEscherichiacoliK-12.http://openwetware.org/wiki/E._coli_genotypes3/142015/5/26E. coli genotypes ­ OpenWetWareEscherichiacoliandSalmonellatyphimurium.CellularandMolecularBiology(Editedby:FCNeidhardtJLIngrahamKBLowBMagasanikMSchaechterHEUmbarger).Washington,D.C.,AmericanSocietyforMicrobiology1987,2:1190-1219.SeeCGSC#1157(http://cgsc2.biology.yale.edu/Strain.php?ID=4509)B2155thrB1004prothistrAhsdsSlacZDM15(F`lacZDM15lacIqtraD36proA+proB+)DdapA::erm(Ermr)pir::RP4[::kan(Kmr)fromSM10]AnE.colistraincarryingthepirsequencerequiredformaintenanceofplasmidscontainingR6Kori.Also,thisstrainisauxotrophicforDAP(diaminopimelicacid-alysineprecursor).Theauxotrophyhelpsinremovalofthisstrainfromabi-parentalmatingsetupafterconjugation.Ref:Maintenanceofbroad-host-rangeincompatibilitygroupPandgroupQplasmidsandtranspositionofTn5inBartonellahenselaefollowingconjugalplasmidtransferfromEscherichiacoliDehio,C.&Meyer,M.(1997)J.Bacteriol.179,538–540BL21+SE.coliBF-dcmompThsdS(rB-mB-)gal[malB]K-12(λ)The"malBregion"wastransducedinfromtheK-12strainW3110tomakethestrainMal+λS.SeeStudieretal.(2009)J.Mol.Biol.394(4),653foradiscussionoftheextentofthetransfer.StratageneE.coliGenotypeStrains(http://www.stratagene.com/pdf/mobio/E%20Coli_Genotype%20Strains.pdf)BL21(AI)–--FompTgaldcmlonhsdSB(rBmB)araB::T7RNAP-tetAanE.coliBstraincarryingtheT7RNApolymerasegeneinthearaBlocusofthearaBADoperonq.TransformedplasmidscontainingT7promoterdrivenexpressionarerepresseduntilL-arabinoseinductionofT7RNApolymerase.MaximalexpressionislowerthanthatofBL21(DE3)(customersupport10/2012)DerivedfromBL21.Seetheproductpage(https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=595)formoreinformation.BrianCaliendo(Voigtlab)reportedtroublegettingtheDatsenkoandWanner(2000)plasmidpCP20totransformintothisstrain,whenotherstrainstransformedfine.Causeisunknown.BL21(DE3)–--FompTgaldcmlonhsdSB(rBmB)λ(DE3[lacIlacUV5-T7gene1ind1sam7nin5])anE.coliBstrainwithDE3,aλprophagecarryingtheT7RNApolymerasegeneandlacIqTransformedplasmidscontainingT7promoterdrivenexpressionarerepresseduntilIPTGinductionofT7RNApolymerasefromalacpromoter.DerivedfromB834(Wood,1966(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=File&DB=pubmed))bytransducingtoMet+.SeetheoriginalStudierpaper(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3537305&query_hl=14)orthesummaryinMethodsinEnzymology(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796&query_hl=13&itool=pubmed_docsum)formoredetails.Wholegenomesequenceavailable[3](http://www.ncbi.nlm.nih.gov/genomes/lproks.cgi)BL21(DE3)pLysS---RFompTgaldcmlonhsdSB(rBmB)λ(DE3)pLysS(cm)pLysSplasmidchloramphenicolresistant;growwithchloramphenicoltoretainplasmidChloramphenicolresistantThepLysSplasmidencodesT7phagelysozyme,aninhibitorforT7polymerasewhichreducesandalmosteliminatesexpressionfromtransformedT7promotercontainingplasmidswhennotinduced.seeMoffatt87fordetailsofpLysSandpLysEplasmidsBNN93---+-FtonA21thi-1thr-1leuB6lacY1glnV44rfbC1fhuA1mcrBe14-(mcrA)hsdR(rKmK)λSomeC600strainsarereallyBNN93BNN97BNN93(λgt11)Aλgt11lysogenproducingphageat42CBW26434,CGSCStrain#7658Δ(araD-araB)567,Δ(lacA-lacZ)514(::kan),lacIp-4000(lacIq),λ-,rpoS396(Am)?,rph-1,Δ(rhaD-rhaB)568,hsdR514ThisinformationisfromaprintoutsentbytheE.coliGeneticStockCenter(http://cgsc.biology.yale.edu)withthestrain.B.L.Wannerstrainrph-1isa1bpdeletionthatresultsinaframeshiftoverlast15codonsandhasapolareffectonpyrEleadingtosuboptimalpyrimidinelevelsonminimalmedium.(Jensen1993JBact.175:3401)http://openwetware.org/wiki/E._coli_genotypes4/142015/5/26E. coli genotypes ­ OpenWetWareΔ(araD-araB)567wasformerlycalledΔaraBADAH33byDatsenkoandWannerAm=amber(UAG)mutationReference:DatsenkoandWanner,2000,PNAS,97:6640NOTE:ThispromoterdrivingtheexpressionoflacIwassequencedinthisstrainusingaprimerinmhpR(upstreamoflacI)andaprimerintheoppositeorientationinlacI.Thelacpromoterwasfoundtobeidenticaltowildtype.Thus,the-35sequencewasGCGCAAnotGTGCAAasexpectedwithlacIq.Thereforethisstrain(oratleasttheversionobtainedfromtheE.coliGeneticStockCenter(http://cgsc.biology.yale.edu))doesNOTappeartobelacIq.AccordingtoBarryWanner,thisisanunexpectedresult.-Reshma13:19,5May2005(EDT)"WehavenowconfirmedthatBW25113,BW25141,andBW26434arealllacI+,andnotlacIq.WethankyouforalertingustotheerrorwithrespecttoBW26434.Apparently,thelacIregionwasrestoredtowild-typeinapredecessorofBW25113."(fromBarryWannerNovember18,2005)ThegenotypehasbeencorrectedattheCGSC(http://cgsc2.biology.yale.edu/Strain.php?ID=65553)C600F-tonA21thi-1thr-1leuB6lacY1glnV44rfbC1fhuA1λ-Therearestrainscirculatingwithbothe14+(mcrA+)ande14-(mcrA-)GeneralpurposehostSeeCGSC#3004(http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/11195)References:Appleyard,R.K.(1954)Genetics39,440;Hanahan,D.(1983)J.Mol.Biol.166,577.C600hflA150(Y1073,BNN102)F-thi-1thr-1leuB6lacY1tonA21glnV44λ-hflA150(chr::Tn10)hostforrepressingplaquesofλgt10whenestablishingcDNAlibrariesReferenceYoungR.A.andDavis,R.(1983)Proc.Natl.Acad.Sci.USA80,1194.TetracyclineresistancefromtheTn10insertionCSH50F-λ-araΔ(lac-pro)rpsLthifimE::IS1SeeCGSC#8085(http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/28630)References:Miller,J.H.1972.Expts.inMolec.Genetics,CSH0:14-0;Blomfeldetal.,J.Bact.173:5298-5307,1991.D1210HB101lacIqlacY+DB3.1--rF-gyrA462endA1glnV44Δ(sr1-recA)mcrBmrrhsdS20(rB,mB)ara14galK2lacY1proA2rpsL20(Sm)xyl5Δleumtl1usefulforpropagatingplasmidscontainingtheccdBoperon.gyrA462enablesccdBcontainingplasmidpropagationstreptomycinresistantappearstoNOTcontainlacI(basedonacolonyPCR)--AustinChe16:16,18June2007(EDT)1.BernardPandCouturierM..pmid:1324324.2.MikiT,ParkJA,NagaoK,MurayamaN,andHoriuchiT..pmid:1316444.DH1-+-endA1recA1gyrA96thi-1glnV44relA1hsdR17(rKmK)λparentofDH5αAnHoffman-Berling1100strainderivative(Meselson68)moreefficientattransforminglarge(40-60Kb)plasmidsnalidixicacidresistantReference:MeselsonM.andYuanR.(1968)Nature217:1110PMID4868368.DH5α--+FendA1glnV44thi-1recA1relA1gyrA96deoRnupGΦ80dlacZΔM15Δ(lacZYA-argF)U169,hsdR17(rKmK),λ–AnHoffman-Berling1100strainderivative(Meselson68)PromegaalsolistsphoAnalidixicacidresistantReferences:FOCUS(1986)8:2,9.Hanahan,D.(1985)inDNACloning:APracticalApproach(Glover,D.M.,ed.),Vol.1,p.109,IRLPress,McLean,Virginia.Grant,S.G.N.etal.(1990)Proc.Natl.Acad.Sci.USA87:4645-4649PMID2162051.MeselsonM.andYuanR.(1968)Nature217:1110PMID4868368.DH5αTurbo(NEB)http://openwetware.org/wiki/E._coli_genotypes5/142015/5/26E. coli genotypes ­ OpenWetWareqSF´proA+B+lacI∆lacZM15/fhuA2∆(lac-proAB)glnVgalR(zgb-210::Tn10)TetendA1thi-1∆(hsdS-mcrB)5AlsoknownasNEBTurboT1phageresistantRapidgrowth:visiblecoloniesonagar,~6.5hours;shakingliquidcultureOD600=2.0,~4hoursExpressestheLacrepressorReferences:NewEnglandBiolabs,productcataloguenumberC2984HDH10B(Invitrogen)F-endA1recA1galE15galK16nupGrpsLΔlacX74Φ80lacZΔM15araD139Δ(ara,leu)7697mcrAΔ(mrr-hsdRMS-mcrBC)λ-suitableforcloningmethylatedcytosineoradeninecontainingDNAanMC1061derivative(Casadaban80).PreparecellsforchemicaltransformationwithCCMB80bufferblue/whiteselectionWhileDH10BhasbeenclassicallyreportedtobegalUgalK,thepreliminarygenomesequenceforDH10BindicatesthatDH10B(andbytheirlineagealsoTOP10andanyotherMC1061derivatives)isactuallygalEgalKgalU+.Dcekiert16:37,23January2008(CST)GenomesequenceindicatesthatDH10BisactuallydeoR+.PresumablyTOP10andMC1061arealsodeoR+.StreptomycinresistantleucineauxotrophReferences:Casdaban,M.andCohen,S.(1980)JMolBiol138:179PMID6997493.Grant,S.G.N.etal.(1990)Proc.Natl.Acad.Sci.USA87:4645-4649PMID2162051.E.coliGeneticStockCenter,MC1061Record(http://cgsc2.biology.yale.edu/Strain.php?ID=11225)DH10BGenomeSequencingProject,BaylorCollegeofMedicine(http://www.hgsc.bcm.tmc.edu/projects/microbial/microbial-detail.xsp?project_id=105)Completesequenceisavailable,seeDurfee08,PMID18245285.DH12S(Invitrogen)mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1deoRΔ(ara,leu)7697araD139galUgalKrpsLF'[proAB+lacIqZΔM15]hostforphagemidandM13vectorsusefulforgeneratinggenomiclibrariescontainingmethylatedcytosineoradenineresiduesstreptomycinresistantReferences:Lin,J.J.,Smith,M.,Jessee,J.,andBloom,F.(1991)FOCUS13,96.;Lin,J.J.,Smith,M.,Jessee,J.,andBloom,F.(1992)BioTechniques12,718.DM1(Invitrogen)F-dam-13::Tn9(CmR)dcm-mcrBhsdR-M+gal1gal2ara-lac-thr-leu-tonRtsxRSu0HostforpBR322andothernon-pUC19plasmids;usefulforgeneratingplasmidsthatcanbecleavedwithdamanddcmsensitiveenzymesChloramphenicolresistantPromegalistsasF'notF-Reference:Lorow-MurrayDandBloomF(1991)Focus13:20E.cloni(r)5alpha(Lucigen)fhuA2Δ(argF-lacZ)U169phoAglnV44Φ80Δ(lacZ)M15gyrA96recA1relA1endA1thi-1hsdR17Commoncloningstrain.E.cloni(r)10G(Lucigen)F-mcrAΔ(mrr-hsdRMS-mcrBC)endA1recA1Φ80dlacZΔM15ΔlacX74araD139Δ(ara,leu)7697galUgalKrpsLnupGλ-tonACommoncloningstrain.ResistanttophageT1.E.cloni(r)10GF'(Lucigen)[F´proA+B+lacIqZΔM15::Tn10(TetR)]/mcrAΔ(mrr-hsdRMS-mcrBC)endA1recA1Φ80dlacZΔM15ΔlacX74araD139Δ(ara,leu)76