综述植物化学成分及其代谢物的定性和定量分析

PAGE/NUMPAGESRecentdevelopmentsinqualitativeandquantitativeanalysisofphytochemicalconstituentsandtheirmetabolitesusingliquidchromatography–massspectrometryHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"HaifengWuHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0005#aff0005"\o"Affiliation:a"a,,HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"JianGuoHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0010#aff0010"\o"Affiliation:b"b,,HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"ShilinChenHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0005#aff0005"\o"Affiliation:a"a,,HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"XinLiuHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0015#aff0015"\o"Affiliation:c"c,,HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"YanZhouHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0020#aff0020"\o"Affiliation:d"d,,HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"XiaopoZhangHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0005#aff0005"\o"Affiliation:a"a,,HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"XudongXuHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"aff0005#aff0005"\o"Affiliation:a"a,,aInstituteofMedicinalPlantDevelopment,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,Beijing100193,PRChinabDepartmentofDMPK,AstraZenecaPharmaceuticals,35GatehouseDrive,Waltham,MA02451,USAcBeijingEntry-ExitInspectionandQuarantineBureau,Beijing100026,PRChinadChengduInstituteofBiology,ChineseAcademyofSciences,Chengdu610041,PRChinaCorrespondingauthorat:InstituteofMedicinalPlantDevelopment,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,No.151,MalianwaNorthRoad,HaidianDistrict,Beijing100193,PRChina.Tel.:+861057833296;fax:+861057833296.Received14May2012Revised30August2012Accepted2September2012Availableonline11September2012HYPERLINK"http://dx.doi.org/10.1016/j.jpba.2012.09.004"\t"doilink"http://dx.doi.org/10.1016/j.jpba.2012.09.004,HYPERLINK"http://www.sciencedirect.com/science/help/doi.htm"\t"sdhelp"HowtoCiteorLinkUsingDOIHYPERLINK"http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=936&_eid=1-s2.0-S0731708512004931&_pii=S0731708512004931&_origin=article&_zone=art_page&_targetURL=https%3A%2F%2Fs100.copyright.com%2FAppDispatchServlet%3FpublisherName%3DELS%26contentID%3DS0731708512004931%26orderBeanReset%3Dtrue&_acct=C000070657&_version=1&_userid=6834453&md5=619d07d9a8a6d0ac40e94af72a43a98c"\t"outwardLink"Permissions&ReprintsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"ViewfulltextHYPERLINK"http://www.sciencedirect.com/science?_ob=ShoppingCartURL&_method=add&_eid=1-s2.0-S0731708512004931&_acct=C000228598&_version=1&_userid=10&_ts=1354809421&md5=179715ca8c6647f5da6e08cf91447050"Purchase $31.501.Introduction2.Qualitativeanalysis3.Quantitativeanalysis4.ApplicationofLC–MSinfingerprintinganalysis5.ConclusionsAcknowledgmentsReferencesAbstractOverthepastfewyears,theapplicationsofliquidchromatographycoupledwithmassspectrometry(LC–MS)innaturalproductanalysishavebeendramaticallygrowingbecauseoftheincreasinglyimprovedseparationanddetectioncapabilitiesofLC–MSinstruments.Inparticular,novelhigh-resolutionhybridinstrumentslinkedtoultra-high-performanceLCandthehyphenationsofLC–MSwithotherseparationoranalyticaltechniquesgreatlyaidunequivocalidentificationandhighlysensitivequantificationofnaturalproductsattraceconcentrationsincomplexmatrices.Withtheaimofprovidinganup-to-dateoverviewofLC–MSapplicationsontheanalysisofplant-derivedcompounds,paperspublishedwithinthelatestyears(2007–2012)involvingqualitativeandquantitativeanalysisofphytochemicalconstituentsandtheirmetabolitesaresummarizedinthepresentreview.Afterbrieflydescribingthegeneralcharacteristicsofnaturalproductsanalysis,themostremarkablefeaturesofLC–MSandsamplepreparationtechniques,thepresentpapermainlyfocusesonscreeningandcharacterizationofphenols(includingflavonoids),alkaloids,terpenoids,steroids,coumarins,lignans,andmiscellaneouscompoundsinrespectiveherbsandbiologicalsamples,aswellastraditionalChinesemedicine(TCM)prescriptionsusingtandemmassspectrometer.ChemicalfingerprintinganalysisusingLC–MSisalsodescribed.Meanwhile,instrumentalpeculiaritiesandmethodologicaldetailsareaccentuated.KeywordsLiquidchromatography;Massspectrometry;Medicinalplants;TraditionalChinesemedicine;Fingerprinting抽象在过去的几年中，液相色谱与质谱联用（LC-MS）在天然产物分析的应用都得到了显着增长，因为日益提高的分离和检测能力的LC-MS仪器。特别是，新颖的高分辨率混合工具与其他分离或分析技术链接到超高性能LC的LC-MS和断字大大有助于在复杂基质中的微量浓度的明确识别和天然产品的高度敏感的定量。提供最新的植物性​​化合物的分析概述LC-MS应用的目的，论文发 表 关于同志近三年现实表现材料材料类招标技术评分表图表与交易pdf视力表打印pdf用图表说话 pdf 在最近几年（2007-2012年），涉及的植物化学成分及其代谢物的定性和定量分析总结在本次审查中。在简要说明天然产物的分析，最显着的特点LC-MS和样品制备技术的一般特点，本论文主要集中在酚类物质（包括类黄酮），生物碱，萜类化合物，类固醇，香豆素，木脂素类化合物的筛选和鉴定，和杂项在各自的草药化合物和生物样品，以及中国传统医学（中医）处方使用串联质谱仪。化学指纹分析用LC-MS也被描述。同时，仪器的特点和 方法 快递客服问题件处理详细方法山木方法pdf计算方法pdf华与华方法下载八字理论方法下载 的细节突出。Figuresandtablesfromthisarticle:Fig.1. Chemicalstructuresofcagedxanthones(1–15)identifiedfromG.hanburyi.ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0445#bib0445"[89].氧杂蒽酮的化学结构（1-15）确定HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0005#fig0005"\o"Viewinworkspace"ViewinworkspaceFig.2. UHPLC–MSchromatogramsofthestandardxanthonesandthecrudeextractsofG.hanburyi.(A)UHPLCchromatogramsof12standardxanthones,(B)TICofthe12standardxanthones,(C)UHPLCchromatogramsofthecrudeextractsofG.hanburyi,(D)TICofthecrudeextractsofG.hanburyi.。2。UHPLC-MS色谱图的 标准 excel标准偏差excel标准偏差函数exl标准差函数国标检验抽样标准表免费下载红头文件格式标准下载 呫吨酮和G.hanburyi粗提物中。（A）（B）UHPLC色谱图的12个标准氧杂蒽酮，TIC的12个标准氧杂蒽酮（C）UHPLC色谱G.hanburyi粗提物中，（D）议会的粗提物的G.hanburyi。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0445#bib0445"[89].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0010#fig0010"\o"Viewinworkspace"ViewinworkspaceFig.3. (A)MS/MSspectraofisogambogenin(13)ofselected[M + H]+atm/z615,(B)MS/MS/MSspectraofselectedionatm/z491fromm/z615,(C)MS/MSspectraofisomorellinol(14)ofselected[M + H]+atm/z547,(D)MS/MS/MSspectraofselectedionatm/z461fromm/z547,(E)MS/MSspectraofcompound15ofselected[M + H]+atm/z545,(F)MS/MS/MSspectraofselectedionatm/z461fromm/z545(collisionenergyat30 eV).图。3。（A）MS/，MS谱isogambogenin（13）选定的[M+H]+m/z为615，（B）选择离子从m/z615，m/z为491MS/MS/MS谱（C）MS/MS，光谱isomorellinol（14）选择[M+H]+m/z为547（D）MS/MS/MS谱的选择离子为m/z461M/Z547，（E）MS/MS谱的化合物15，所选的[M+H]+为m/z545（F）MS/MS/MS谱图，从m/z545（碰撞能量30eV的选择离子为m/z461）。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0445#bib0445"[89].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0015#fig0015"\o"Viewinworkspace"ViewinworkspaceFig.4. Proposedfragmentpathwaysof[M + H]+for:(A)isogambogenin(13),(B)isomorellinol(14),(C)15.ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0445#bib0445"[89].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0020#fig0020"\o"Viewinworkspace"ViewinworkspaceFig.5. HSCCCchromatogramofgambogeextract(190 mg).FractionI(17.26 mg),fr.II(5.48 mg),fr.III(3.81 mg),fr.IV(3.56 mg),fr.V(7.66 mg),fr.VI(3.21 mg),fr.VII(8.21 mg),fr.VIII(35.25 mg),fr.IX(76.78 mg),fr.X(6.04 mg),fr.XI(4.34 mg).ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0460#bib0460"[92].图。4。已提议片段途径的[M+H]+为：（A）isogambogenin（13），（B）isomorellinol（14），（C）15。转载许可[89]。HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0025#fig0025"\o"Viewinworkspace"ViewinworkspaceFig.6. SchemeoftheHPLCon-linecoupledtoESI-MSandDPPHassay.Thearrowsindicateflowdirections.图。6。 计划 项目进度计划表范例计划下载计划下载计划下载课程教学计划下载 的HPLC上线耦合到：ESI-MS法和DPPH。箭头表示流动方向。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0475#bib0475"[95].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0030#fig0030"\o"Viewinworkspace"ViewinworkspaceFig.7. TheoverviewoftheNMR/LC–MSparalleldynamicspectroscopy(NMR/LC–MSPDS)combinedwithanuncompletedseparationstrategyforsimultaneousstructureidentificationofnaturalproductsincrudeextract.图。7。未完成的同时进行结构鉴定天然产物粗提物中分离策略，结合NMR/LC-MS，并行动态光谱仪（NMR/LC-MSPDS）的概述。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0480#bib0480"[96].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0035#fig0035"\o"Viewinworkspace"ViewinworkspaceFig.8. Proposedmetabolicpathwaysforhumanliverphase1metabolismofisoliquiritigenin.图。8。建议第1阶段人类肝脏代谢，异甘草素的代谢途径。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0500#bib0500"[100].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0040#fig0040"\o"Viewinworkspace"ViewinworkspaceFig.9. TheschematicdiagramofVEGFR-CMC-on-line-LC–MSsystem.UV:ultravioletdetector;DAD/MS:diodearraydetector/massspectrometerdetector;1DCMCcolumn:theVEGFR-CMCcolumnplayingasthefirstdimensionalcolumn.2Danalyticalcolumn:theVP-ODScolumnplayingastheseconddimensionalcolumn.图。9。VEGFR-CMC-线LC-MS系统的示意图。UV：紫外检测器，DAD/MS：二极管阵列检测/质谱法等检测;一维CMC柱中：VEGFR-军委纵队打的第一维列。二维分析柱：VP-ODS柱打的第二维列。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0545#bib0545"[109].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0045#fig0045"\o"Viewinworkspace"ViewinworkspaceFig.10. Frameworkforhigh-throughputscreeningofcardio-protectivecompoundsfrommedicinalplantextracts.。10。从药用植物提取物的保护心血管的化合物的高通量筛选的框架。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0775#bib0775"[155].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0050#fig0050"\o"Viewinworkspace"ViewinworkspaceFig.11. UHPLC–QTOFbasepeakintensity(BPI)chromatogramsofsixMeconopsisspeciesforidentification:(a–f)BPIofESI(+)TOF-MSfromM.torquata;M.racemosa;M.integrifolia;M.quintuplinervia;M.betonicifolia;M.horridula.图。11。UHPLC-QTOF基峰强度（BPI）色谱绿绒蒿物种识别：（A-F）BPI的ESI（+）TOF-MS从M.torquata;M.鹃，M.integrifolia，M.quintuplinervia，;M.betonicifolia;M.horridula。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0875#bib0875"[175].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0055#fig0055"\o"Viewinworkspace"ViewinworkspaceFig.12. StructuresassignedintheextractsofMeconopsisspecies.图。12。绿绒蒿属物种的提取物中分配的结构。ReproducedwithpermissionfromHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"bib0875#bib0875"[175].HYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"#"FigureoptionsHYPERLINK"http://www.sciencedirect.com/science/article/pii/S0731708512004931"\l"fig0060#fig0060"\o"Viewinworkspace"Viewinworkspace友情提示： 方案 气瓶 现场处置方案 .pdf气瓶 现场处置方案 .doc见习基地管理方案.doc关于群访事件的化解方案建筑工地扬尘治理专项方案下载 范本是经验性极强的领域，本范文无法思考和涵盖全面，供参考!最好找专业人士起草或审核后使用。