测序通用引物序列

测序通用引物序列 常见载体的测序引物: Primer of Vector: Primer(F);Primer(R) Vector: pACT T7 T3 pACT2 GAL4 AD pACT2-R pAS2-1 GAL4 BD pAS2-1.R pB42AD pB42ADF pB42ADR pBACPAK8 BAC1 BAC2 pBK-CMV T7 T3 PBS(SK/KS)/M13- M13F/T7 T3/M13R pBV220 PBV220F PBV220R pCAMBIA 1301(1300) P1 P2 pCAMBIA 2300 M13R(-48) M13F(-47) PCANTB5E S1/M13R S6 pCAT3-enhancer RVP3 此载体无反向引物 pcDNA3.0 CMV-F/T7 SP6/BGH pcDNA3.1 T7 BGH pcDNA4 T7/CMV-F BGH pcDNA6 T7/CMV-F(J21025在T7前面) BGH pcDNAII T7 SP6 pCE2.1 M13F M13R pCEP4 pCEP-F EBV-R pCF-T M13F M13R pCI T7(17Base) 此载体无反向引物 pCI-neo T7(17Base) T3 pCMS-EGFP T7 T3 pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5F pCMV5R pCMV-MYC/HA pCMV-F pCMV-R pCMV-Sport M13F/T7 SP6/M13R pCMV-Tag(KAN+) T3 T7 pCR2.1-TOPO M13F/T7 M13R pCR3.1 T7 BGH pCS2 SP6 T7 pDonar M13F M13R pDONR221 M13F M13R pDrive T7 SP6 pDRIveR(KAN+) T7 SP6 pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用) pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’ pDSRED-N1(KAN+) pEGFP-N-5’ PDSRED-N-R pECFP-C pEGFP-C-5’ pECFP-C-3′ pECFP-N1 pEGFP-N-5’ pEGFP-N-3’ pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C(KAN+) pEGFP-C-5’ pEGFP-C-3′ pEGFP-N(KAN+) pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO(KAN+) M13F M13R pET-*(His)(KAN+) T7 T7 Ter pET22(a,b,c)(AMP+) T7 T7 Ter pET28,30,24,49(KAN+) T7 T7 Ter pET32(a,b,c)(AMP+) T7/S.tag T7 Ter pET50(amp+) T7/s.tag T7TER pET44(AMP+) T7/s.tag T7 Ter pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pEYFP-C1 pEGFP-C-5’ pEGFP-C-3′ pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -R pGAD424 GAL4 AD 3′AD pGADGH GAL4 AD 3′AD pGADT7-Rec GAL4 AD/T7 3′AD pGAPZa-A a-FACTOR 3′AOX pGBKT7(Kana+) T7 3′BD pGEM-T(-Easy) M13F/T7 SP6/M13R pGEX-(*)T pGEX-4T-5’ pGEX-4T-3’ pGL2 GLP1 GLP2 pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2) PinpointTM Vector Pinpoint Primer SP6 pIRES PIRES-F/T7 T3/PIRES-R (J40720) pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’ pLXSN pLXSN-F pLXSN-R pMAL-c2E MalE M13F(-47) pMAL-C2x P5’ P3’ pMAL-p2X MalE M13F(-47) PMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48) pPIC9K(AMP+、ZEO+) 5’AOX/a-factor 3’AOX pPICZa 5′AOX/PPICZa-F 3′AOX pPROEXHTA M13R(-48) 无反向引物 pQE30or40 pQE30-F pQE-R: pRSET T7 T7TER pSilencer3.1-H1 hygro M13F(-47) 3.0REV pSILENCEV2.0-U6 T7 2.0REV pSK01-T M13F M13R pSK-CMV(KAN+) T7 T3 pSP72 T7 SP6 pSport1 SP6/M13F(-47) T7/M13R pSUPER T7 T3 pTA2 M13F/T7(优先使用M13F) T3/M13R pT-Adv M13F /T7 M13R pTARGET TM pTarget.F(在T7前面)/T7 pTarget.R pTO-T7 T7 T7TER pTRC99a-c pTRC99C-F pTRC99C-R pTriPLEx2 5′pTriPLEx2 T7 pTWIN-1 T7 T7TER(客户确认再用) pUC18(19)/118(119) M13F(-47) M13R(-48) pUCm-T M13F/T7 M13R pVAX1 T7 BGH pWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48) pXT7 T7 SP6 pYES2 T7 pYes2.R pLEXA pLEXA-F pLEXA-R pLNCX pLNCX-F pLNCX-R pRECEIVER CMV-F 此载体无反向引物 pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSP64/65 SP6 此载体无反向引物 pSTBlue-1 T7 SP6 pT7Blue(R) T7 M13F(-47) 引物名称 序列(5′-3′): M13R:CAG GAA ACA GCT ATG ACC M13F:TGT AAA ACG ACG GCC AGT M13F(-47):CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48):AGC GGA TAA CAA TTT CAC ACA GGA M13(-96):CCC TCA TAG TTA GCG TAA CG SP6:ATT TAG GTG ACA CTA TAG T7:TAA TAC GAC TCA CTA TAG GG T7 terminator:TGC TAG TTA TTG CTC AGC GG T3:ATT AAC CCT CAC TAA AGG GA pGEX-4T-5′:GGG CTG GCA AGC CAC GTT TGG TG pGEX-4T-3′:CCG GGA GCT GCA TGT GTC AGA GG GLp1:TGT ATC TTA TGG TAC TGT AAC TG GLp2:CTT TAT GTT TTT GGC GTC TTC CA RVp3:CTA GCA AAA TAG GCT GTC CC RVp4:GAC GAT AGT CAT GCC CCG CG pcDNA3.1R:TAG AAG GCA CAG TCG AGG PinPoint : CGT GAC GCG GTG CAG GGC G pCMV-F: TCT AAA AGC TGC GGA ATT GT pCMV-R: TCCAAACTCATCAATGTATC pTRC99C-F: TTG CGC CGA CAT CAT AAC pTRC99C-R: CTGCGTTCTGATTTAATCTG pCEP-F: AGA GCT CGT TTA GTG AAC CG EBV-R : GTG GTT TGT CCA AAC TCA TC pIRES2-EGFP.P5’: GTA GGC GTG TAC GGT GGG AG pIRES2-EGFP.P3’: AAC GCA CAC CGG CCT TAT TC 3′AD: AGA TGG TGC ACG ATG CAC AG CMV -F: CGC AAA TGG GCG GTA GGC GTG S1: CAA CGT GAA AAA ATT ATT ATT CGC S6: GTA AAT GAA TTT TCT GTA GTA GG 5`AOX1:GAC TGG TTC CAA TTG ACA AGC 3`AOX1:GCA AAT GGC ATT CTG ACA TCC α-Factor:TAC TAT TGC CAG CAT TGC TGC GAL4 AD:TAC CAC TAC AAT GGA TG pACT2-R:GTGCACGATGCACAGTTGAA pB42ADF:CCA GCC TCT TGC TGA GTG GAG ATG pB42ADR:AAG CCG ACA ACC TTG ATT GGA G pEGFP-N-5’:TGG GAG GTC TAT ATA AGC AGA G pEGFP-N-3’:CGT CGC CGT CCA GCT CGA CCA G pEGFP-C-5’: CAT GGT CCT GCT GGA GTT CGT G pEGFP-C-3′ :TAT GGC TGA TTA TGA TCA GT PBV220F:AAG AAG GGC AGC ATT CAA AG PBV220R:CTG CGT TCT GAT TTA ATC TG U6:ATG GAC TAT CAT ATG CTT ACC GTA 2.0rev primer:AGG CGA TTA AGT TGG GTA 3.0rev:GAG TTA GCT CAC TCA TTA GGC GAA CGC CAG CAC ATG GAC S.tag: 5′TriplEx2:CTC CGA GAT CTG GAC GAG C RVP4:GAC GAT AGT CAT GCC CCG CG RVP3:CTA GCA AAA TAG GCT GTC CC pQE30-R:GTT CTG AGG TCA TTA CTG G pQE30-F:TGA GCG GAT AAC AAT TTC AC pEF-F:TCA AGC CTC AGA CAG TGG TTC pDSRED-N1-R:TGA AGC GCA TGA ACT CCT TG pDsRED-express-C1-F:TCC CAC AAC GAG GAC TAC AC pCMV5R: ATT ATA GAG GAC ACC TAG TC pCMV5F: TTC CAA AAT GTC GTA ATA AC P5′: TGC GTA CTG CGG TGA TCA AC P3′: CTG CAA GGC GAT TAA GTT GG BAC2: ACG CAC AGA ATC TAG CGC TT BAC1: AAC CAT CTC GCA AAT AAA TA 3′BD: TAA GAG TCA CTT TAA AAT TTG TAT AC pTarget.F: CCA GGA TTT TCC CAG TCA C pTarget.R: GGC TTT ACA CTT TAT GCT TC T7(17base): ACA TCC ACT TTG CCT TTC TC pYes2.R: TCG GTT AGA GCG GAT GTG GAL4 BD: TCA TCG GAA GAG AGT AG pAS2-1.R: AAG AGT TAC TCA AGA ACA AGA A pFlag-CMV-F: GGT AGG CGT GTA CGG TGG pFlag-CMV-R: GCA CTG GAG TGG CAA CTT pIRES-F: CTT ACT GAC ATC CAC TTT GC pIRES.R: CAC TGC ATT CTA GTT GTG GT P1: CCA GGC TTT ACA CTT TAT GC P2: GCG ATT AAG TTG GGT AAC GC pLEXA-F: CGT CAG CAG AGC TTC ACC AT pLEXA-R: TAA AAC CTA AGA GTC ACT TT pLNCX-F: AGC TCG TTT AGT GAA CCG TCA GAT CG pLNCX-R: ACC TAC AGG TGG GGT CTT TCA TTC CC pLXSN-F: CTT GAA CCT CCT CGT TCG AC pLXSN-R: GTT GCT GAC TAA TTG AGA TG pSHUTTLE-CMV-F: GGT CTA TAT AAG CAG AGG TG pSHUTTLE-CMV-R: GTG GTA TGG CTG ATT ATG ATC AG MalE Primer: GGT CGT CAG ACT GTC GAT GAA GCC