孕妇羊水afp的MATCH_
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_1714413818813_0价值分析
孕妇羊水AFP的检测价值分析
孕妇羊水AFP的检测价值分析
【摘要】 目的: 探讨孕妇年龄因素对孕中期(16周)羊水甲胎蛋
白(AFP)浓度的影响。
方法
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: 采用电化学发光法,测定20051~月
来我院检查的怀孕16周孕妇羊水AFP浓度,将测定值按孕妇年龄分
组(G1组、G2组、G3组)进行比较分析。结果: 羊水AFP浓度随
孕妇年龄增大而升高(P005),且4例确诊胎儿神经管缺陷孕妇年龄
均大于30岁。结论: 孕中期(16周)孕妇羊水AFP浓度受年龄因
素影响,高龄孕妇羊水AFP浓度明显高于适龄孕妇。提示:高龄孕妇
应做羊水AFP筛查检测,有助于提高神经管缺陷患儿的诊出率,避免
患儿出生。 【关键词】 羊水;甲胎蛋白;神经管缺陷 【ABSTRACT】
Objective: To study the influence of the pregnant womans age on the AFP in amniotic fluid during the 16th GW. Methods: The AFP in amniotic fluid was checked with ECLIA mothod for pregnant wowen in 2005, and all the abtained data were divided into three groups(in turn G1 group,G2 group,G3 group)according to the
pregnant wowans age. Results: The AFP density of the older group was higher than that of the younger group ( P005 ), four gregnant women who were diagnosed of nerve tube blemish were all over 30 years. Conclusion: The AFP density in amniotic fluid during the 16th GW will be influenced with the increasing of prenant womans age, the AFP density of the older group was higher than that of the younger group. Hint: For the older pregnant woman, the AFP in amniotic fluid should be checked as a rule.The check rate of nerve tube blemish can be increased,and the birth of
ill infant can be avoided. 【KEY WORDS】 Amniotic fluid; AFP;
Nerve tube blemish 甲胎蛋白(alpha fetoprotein,AFP)是一种
分子量为70000的糖蛋白。在胎儿期,AFP主要在肝脏及卵黄囊中合
成,并释放入胎儿血液、脑脊液中。AFP经胚胎途径可到达母亲血液,
随胎尿排入羊水。在怀孕周羊水AFP开始增高,16~18周达到高峰,
以后逐渐下降。胎儿发生神经管缺陷时,AFP经开放的神经管进入羊
水导致羊水AFP浓度显着升高[1]。我们对20051~月来我院检查的孕妇,根据知情自愿原则,对580名孕妇抽取羊水进行AFP浓度测定,并进行分组比较。 1
资料
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和方法 11 一般资料 将接受羊水AFP浓度检测的580名孕16周的孕妇,按孕妇年龄分为3组,其中30岁以下(G1组)91例,30~35岁(G2组)246例,35岁以上(G3组)243例。 试剂及仪器 罗氏公司 Elecsys 电化学发光仪及罗氏AFP检测试剂盒。质控品、校正品也均由罗氏公司提供。 13 方法 抽取孕妇羊水3ml,取500μl羊水装入标本杯,仪器设置稀释倍数为50,在波长620nm处采用电化学发光法检测羊水AFP浓度,各实验均严格按照操作规程进行操作。 14 统计分析 测量数据先经对数转换,再采用均数+
标准
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差(x?s)进行t检验。 2 结果 21 羊水AFP浓度随孕妇年龄增大而升高,孕中期(16周)高龄孕妇羊水AFP浓度明显高于适龄孕妇(P005)。各组羊水AFP浓度见表1。表1 三个年龄组羊水AFP比较(略)注:* P005,G2、G3组分别与G1组比较;? P005,G3组与G2组比较