通用引物、常用引物及序列对照

通用引物、常用引物及序列对照 General Primers BGHrev TAGAAGGCACAGTCGAGG GFPfusionREV CTCCACTGACAGAAAATTTG M13 (-21) Forward TGTAAAACGACGGCCAGT M13 (-40) GTTTTCCCAGTCACGAC M13 Reverse CAGGAAACAGCTATGAC MSCV-rev CAGCGGGGCTGCTAAAGCGCATGC pBluescriptSK TCTAGAACTAGTGGATC pBluescriptKS TCGAGGTCGACGGTATC pGEX3' CCGGGAGCTGCATGTGTCAGAGG pGEX5' GGGCTGGCAAGCCACGTTTGGTG SP6 ATTTAGGTGACACTATAG T7 Terminal GCTAGTTATTGCTCAGCGG Universal T3 GCAATTAACCCTCACTAAAGG Universal T7 TAATACGACTCACTATAGGG Primers from Invitrogen 3'AOX1 GCAAATGGCATTCTGACATCC 5'AOX1 GACTGGTCCAATTGACAAGC AC5 ACACAAAGCCGCTCCATCAG AUG1 Forward CAATTTACATCTTTATTTATTAACG AUG1 Reverse GAAGAGAAAAACATTAGTTGGC BGH Reverse TAGAAGGCACAGTCGAGG Baculovirus Forward TTTACTGTTTTCGTAACAGTTTTG CMV Forward CGCAAATGGGCGGTAGGCGTG CYC1 GCGTGAATGTAAGCGTGAC EBV Reverse GTGGTTTGTCCAAACTCATC EF-1a Forward TCAAGCCTCAGACAGTGGTTC Ecdysone Forward CTCTGAATACTTTCAACAAGTTAC GAL1 AATATACCTCTATACTTTAACGTC M13/pUC Forward CCCAGTCACGACGTTGTAAAACG M13/pUC Reverse AGCGGATAACAATTTCACACAGG Metallothionein CATCTCAGTGCAACTAAA OpIE2 Forward CGCAACGATCTGGTAAACAC V5 Reverse ACCGAGGAGAGGGTTAGGAT Xpress Forward TATGGCTAGCATGACTGGT alpha-factor TACTATTGCCAGCATTGCTGC cI Forward GGATAGCCGGTCAGGTGTT pBAD Forward ATGCCATAGCATTTTTATCC pBAD Reverse GATTTAATCTGTATCAGG pCEP Forward AGAGCTCGTTTAGTGAACCG pGAP GTCCCTATTTCAATCAATTGAA pGENE Forward CTGCTATTCTGCTCAACCT pHybLex Forward AGGGCTGGCGGTTGGGGTTATTCGC pHybLex Reverse GAGTCACTTTAAAATTTGTATACAC pJG4-5 Forward GATGCCTCCTACCCTTATGATGTGCC pJG4-5 Reverse GGAGACTTGACCAAACCTCTGGCG pREP Forward GCTCGATACAATAAACGCC pRH Forward CTGTCTCTATACTCCCCTATAG pRH Reverse CAAAATTCAATAGTTACTATCGC pTrcHis Forward GAGGTATATATTAATGTATCG pTrcHis Reverse GATTTAATCTGTATCAGG pUni Forward CTATCAACAGGTTGAACTG pUni Reverse CAGTCGAGGCTGATAGCGAGCT pYESTrp Forward GATGTTAACGATACCAGCC pYESTrp Reverse GCGTGAATGTAAGCGTGAC Polyhedrin forward AAATGATAACCATCTCGC Polyhedrin reverse GTCCAAGTTTCCCTG Primers from BD Biosciences 3'AOX1 GCAAATGGCATTCTGACATCC EGFP-C CATGGTCCTGCTGGAGTTCGTG EGFP-N CGTCGCCGTCCAGCTCGACCAG GAL1 AATATACCTCTATACTTTAACGTC Gal4-AD AATACCACTACAATGGAT HA ATGTACCCATACCATGTTCCAGATTAC HAT GAGGAGCACGCTCATGCCCAC LNCX AGCTCGTTTAGTGAACCGTCAGATC MSCV CCCTTGAACCTCCTCGTTCGACC Myc GCATCAATGCAGAAGCTGATCTCA DsRed1-N GTACTGGAACTGGGGGGACAG DsRed1-C AGCTGGACATCACCTCCCACAACG pCMV GATCCGGTACTAGAGGAACTGAAAAAC Other Primers Bglob-intron-F CTGGTCATCATCCTGCCTTT Bglob-pA-R TTTTGGCAGAGGGAAAAAGA EXFP-R GTCTTGTAGTTGCCGTCGTC (Golenbock lab) GFP-F GGTCCTTCTTGAGTTTGTAAC GFP-R CCATCTAATTCAACAAGAATTGGGACAAC (Ahmad lab) Gal10pro-F GGTGGTAATGCCATGTAATATG (Stratagene) Gal4 N-term GAGTAGTAACAAAGGTCAA GPDpro-F CGGTAGGTATTGATTGTAATTCTG H1 TCGCTATGTGTTCTGGGAAA HA-F TACCCATACGACGTCCCAGA HA-R TCTGGGACGTCGTATGGGTA hGH-PA-R CCAGCTTGGTTCCCAATAGA IRES-R CCTCACATTGCCAAAAGACG L4440 AGCGAGTCAGTGAGCGAG (Caldwell lab) LacZ-R GACAGTATCGGCCTCAGGAA LexA CGTCAGCAGAGCTTCACCATTG (Caldwell lab) LKO.1 5' GACTATCATATGCTTACCGT (Weinberg Lab) LucNrev CCTTATGCAGTTGCTCTCC MBP-F GATGAAGCCCTGAAAGACGCGCAG (Waugh lab) mPGK-F CATTCTGCACGCTTCAAAAG MT1-F GCTGTCCTCTAAGCGTCACC mU6-F CAGCACAAAAGGAAACTCACC NOS-F GCGTTCAAAAGTCGCCTAAG pACLU AGAGCCTGATAAAAACGGTTAGCG (Joung lab) pBABE 3' ACCCTAACTGACACACATTCC (Weinberg Lab) pBABE 5' CTTTATCCAGCCCTCAC (Weinberg Lab) pBAC CGCCAGGGTTTTCCCAGTCACGAC (Joung lab) pBMN 5' GCTTGGATACACGCCGC pBRforBam CTTGGAGCCACTATCGAC pBRrevBam GGTGATGTCGGCGATATAGG pCasper-F GGGTTTTATTAACTTACAT (Vosshall lab) pCAG-F GCAACGTGCTGGTTATTGTG pcDL-F GTTGCCTTTACTTCTAGGCCT (Kinet lab) pENTR-F CTACAAACTCTTCCTGTTAGTTAG pGP GGGGGATTTCTGTTCATGGGGG (Joung lab) pGP704-R AACAAGCCAGGGATGTAACG pGV-2U ACTAACATACGCTCTCCATCAA (Yamanaka lab) pLTet-F ACTGAGCACATCAGCAGGAC pLXSN 5' CCCTTGAACCTCCTCGTTCGACC (MSCV) pMT2-F TTGCCTTTCTCTCCACAGGT pMX-S1811 GACGGCATCGCAGCTTGGATACAC (Yamanaka lab) puro-F GCAACCTCCCTTCTACGAGC RCAS-F ACATGGGTGGTGGTATAGCGCTTGCG (Orsulic lab) RVprimer3 CTAGCAAAATAGGCTGTCCC (Promega) SFFV-F ATTGATTGACTGCCCACCTC SV40pA-R GAAATTTGTGATGCTATTGC SV40pro-F TATTTATGCAGAGGCCGAGG sv40-spliceR CACAAAGATCCGGACCAAAG tac promoter GAGCGGATAACAATTTCACACAGG (Waugh lab) TRC-F CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Ubx-F AACTCGTACTTTGAACAGGC WPRE-R CATAGCGTAAAAGGAGCAACA