卵母细胞论文：卵母细胞 卵胞浆内单精子显微注射 第一极体 胚胎

卵母细胞 论文 政研论文下载论文大学下载论文大学下载关于长拳的论文浙大论文封面下载 ：卵母细胞 卵胞浆内单精子显微注射 第一极体 胚胎 卵母细胞论文:卵母细胞 卵胞浆内单精子显微注射 第一极体 胚胎 【中文摘要】人类辅助生殖技术(Assisted reproductive technology, ART)在治疗不孕不育症的过程中取得了很大的进展,而卵胞浆内单精子显微注射(instracytoplasmis sperm injection, ICSI)作为治疗男性严重少弱精症的主要措施,已经成为一项重要的辅助生殖技术。影响ICSI结局的因素中以卵子质量最为重要,可以通过两方面对卵子质量进行评价:胞浆发育和核发育。胞浆发育程度主要通过对胞浆颗粒的颜色、空泡(Vacuole)、包涵体(inclusion body)、折光体(inclusion body)等进行评价。而核发育程度可以通过第一极体的形态进行评价。在ICSI过程中对卵母细胞胞浆和核进行评价,从而判断卵子质量,指导植入胚胎选择,改善ICSI结局。1.了解不同类型第一极体人卵母细胞与三种来源精子经ICSI后正常受精率、卵裂率和优质胚胎率的关系。2.了解人卵母细胞胞浆状态与ICSI后正常受精率、卵裂率和优质胚胎率的关系。方法选择2009年2月至2009年8月在郑大三附院生殖医学中心行ICSI治疗的110个周期中1017枚M?期人卵母细胞。?按精液来源不同将卵母细胞分为三组:手淫取精组、附睾穿刺/睾丸活检(PESA/TESA)取精组、冷冻复苏精液组;?按卵母细胞第一极体形态不同分为?、?、?、?四种类型;?再按卵母细胞第一极体完整程度分为完整规则型第一极体和非完整规则型第一极体卵母细胞;?按卵母细胞内是否含有特殊形 态结构分为:无特殊结构卵子组和含有空泡、折光体、滑面内质网(smooth endoplasmic reticulum, SER)小囊聚集体、卵黄膜异常、卵胞间隙异常卵子组。通过卵子准备、精子准备和ICSI操作过程,分析相关数据:1.三种不同精液来源组中卵母细胞的四种类型第一极体经ICSI后正常受精率、卵裂率和优质胚胎率的关系。2.完整规则型第一极体与非完整规则型第一极体卵母细胞经ICSI后正常受精率、卵裂率和优质胚胎率的关系。3.无特殊结构卵子组与含有空泡、折光体、滑面内质网聚集体、卵黄膜异常、卵胞间隙异常卵子组经ICSI后正常受精率、卵裂率和优质胚胎率的关系。所有数据采用SPSS16.0统计软件包处理。计数资料采用χ2检验。检验水准为a=0.05。结果1.手淫取精组中?型极体卵母细胞正常受精率和?型极体卵母细胞正常受精率分别为76.33%(158/207)和63.87% (244/382),两组比较有显著性差异(χ2=9.610,P<0.05)。2.完整规则型第一极体卵母细胞与非完整规则型第一极体卵母细胞正常受精率分别为70.80% (337/476)和64.70% (350/541),两组比较有显著性差异(χ2=4.303,P<0.05)。3.无特殊结构卵子组与含特殊结构卵子组的正常受精率分别为71.93%(551/766)和54.18%(136/251),两组比较有显著性差异(χ2=27.170,P<0.05)。4.无特殊结构卵子组正常受精率71.93% (551/766)与卵胞间隙异常卵子组正常受精率50%(14/28)、空泡异常卵子组正常受精率44.44%(8/18)、卵黄膜异常卵子组正常受精率38.38%(38/99)分别比较,有显著性差异(χ2=6.331,P<0.05;χ2=6.494,P<0.05;χ2=45.415,P<0.05)。5.无特殊结构卵子组与含 特殊结构卵子组第三天优质胚胎率分别为59.54%(312/524)和 44.27%(58/131),两组比较有显著性差异(χ2=9.938,P<0.05)。6.无 特殊结构卵子组与卵黄膜异常卵子组的第三天优质胚胎率分别为 54.63% (283/518)和28.57%(10/35),两组比较有显著性差异(χ 2=14.942,P<0.05)。结论1.第一极体完整且规则的卵母细胞经ICSI 后能够更好的完成受精。2.无特殊结构卵母细胞经ICSI后能够更好 的完成受精。3.卵母细胞卵黄膜张力差,形态不规则可直接导致ICSI 后胚胎退化或发育不良。 【英文摘要】Human Assisted Reproductive Technology (ART) process in the treatment of infertility has made great progress and intracytoplasmis sperm injection (ICSI) as a treatment for less severe asthenozoospermia men the main measures has become an important assisted reproductive technology. The most important factors that affect the outcome of ICSI is the quality of ovum, we can evaluate it by two aspects: cytoplasmic development and nuclear growth. Mainly the degree of cytoplasmic development can be evaluated through the state of the contents of cytoplasm. The level of nuclear development can be evaluated it through the emergence of the first polar body. In the ICSI process, we can through the color of cytoplasmic particlesm, size of the vacuoles, Vacuole, inclusion body, inclusion body, first polar body morphology and so on to judge the quality of ovum and to guide the selection of implantation embryo for the outcome of the improvement of ICSI.1、To understand the relationship of different types of first polar body of human oocytes and normal fertilization rate, cleavage rate and high quality embryo rate after ICSI using three sources of sperm 2、To understand the relationship of the state of cytoplasm of human oocytes and normal fertilization rate cleavage rate and high quality embryo rate after ICSI.MethodsMethod of retrospective study that 1017 M?of human oocytes in 110 cycles of ICSI from February 2009 to August 2009.?the oocytes were divided into three groups by the sources of different Semen:masturbation sperm retrieval group, PESA/TESA sperm retrieval group, frozen semen group;?First polar body morphology were divided into four types;?According to the shape of eggs were divided into two groups:the group one is normal eggs and another is abnormal.?Oocytes by the presence of a special form within the structure is divided into two groups:no special structure egg group and containing the egg cavity, refractive body, smooth endoplasmic reticulum (SER) aggregates of small sacs, the yolk membrane abnormalities, abnormal egg cell gap egg group:1、The relationship of four types of oocytes first polar body from three different groups of semen after ICSI normal fertilization rate after cleavage rate and high quality embryo rate.2、The relationship between group of normal egg and group of abnormal egg normal fertilization rate, cleavage rate and high quality embryo rate after ICSI.3、The relationship between group of no special structure egg group and containing the egg cavity, refractive body, smooth endoplasmic reticulum (SER) aggregates of small sacs, the yolk membrane abnormalities, abnormal egg cell gap egg group after ICSI normal fertilization rate after cleavage rate and high quality embryo rate.Statistical Package for all data handled by SPSS 16.0. Count data byχ2 test. Test for the α=0.05.Results1、There was significance comparing respectively normal fertilization rate with polar body type?of oocytes {63.87%(244/382)} and polar body type?of oocytes {76.33%(158/207)} in retrieval masturbation sperm (χ2=9.610, P< 0.05) 2、There was significance comparing respectively normal fertilization rate with Type the full rules oocytes first polar body rule-based group {70.80%(337/476)} and non-intact oocytes first polar body group {64.70%(350/541)} (χ2=4.303, P <0.05)3、There was significance comparing respectively normal fertilization rate with non-special structure eggs group {71.93% (551/766)} and the eggs with the special structure group {54.18%(136/251)} (χ2=27.170, P<0.05)4、There was significance comparing respectively normal fertilization rate with non-special structure eggs group {71.93% (551/766)} and the abnomal intercellular spaces egg group {50%(14/28)}、abnormal vacuolar egg group {44.44%(8/18)}、abnormal yolk membrane egg group {38.38%(38/99)} (χ2=6.331, P<0.05; X2=6.494, P<0.05;χ 2=45.415, P<0.05)5、There was significance comparing respectively the third day of the quality embryo rate with non-special structure eggs group {59.54%(312/524)} and the eggs with the special structure group {44.27%(58/131)} (χ 2=9.938, P<0.05)6、There was significance comparing respectively the third day of the quality embryo rate with non-special structure eggs group{59.54%(312/524)} and abnormal yolk membrane egg group {28.57%(10/35)} (χ2=14.942, P<0.05)Conclusion1、Complete the first polar body and the rules of the oocytes by ICSI had better fertilization.2、Normal form oocytes after ICSI fertilization can be done better.3、Yolk membrane tension is important structure to maintain the vitality of embryos, the yolk membrane tension difference led a direct result:degradation of embryos or dysplasia. 【关键词】卵母细胞 卵胞浆内单精子显微注射 第一极体 胚胎 【英文关键词】oocytes ICSI the first polar body embryo 【目录】影响人卵母细胞单精子显微注射胚胎结局的相关因素分 摘要 4-7 Abstract 7-9 缩略词 表 关于同志近三年现实表现材料材料类招标技术评分表图表与交易pdf视力表打印pdf用图表说话 pdf 11-12 引析 言 12-13 材料与方法 13-19 结果 19-24 讨论 24-30 结论 30-31 参考文献 31-35 附图1 卵母细胞第一极体形态 35-36 附图2 卵母细胞胞浆异常结构 36-37 综述部分 人类配子在体外授精中的作用 37-47 参考文献 44-47 附录部分 47-48 个人简介 47 简历 个人简历下载免费下载简历模版总经理简历下载资料员简历下载资料员简历下载 47-48 致谢 48 【索购全文找】1.3.9.9.3.8.8.4.8 1.3.8.1.1.3.7.2.1 同时提供论文写作一对一辅导和论文发表服务。 【说明】本文仅为中国学术文献总库合作提供，无涉版权。作者如有异议请与总库或学校联系。