NEB酶切buffer

Home > Tec hnic al Refer enc e > Restriction Endonucleases > NEBuffer A ctivity Chart NEBuffer Activity Chart for Restriction Enzymes For your c onvenienc e, N ew England Biolabs offers a simple 4 buffer system. A c olor-c oded 10X NEBuffer is supplied with every r e endonuclease, ensuring 100% activity. Over 170 r estriction enzymes exhibit 100% activity in NEBuffer 4, r esulting in increase d eff and ease of use especially when per for ming double digests. To help select the best c onditions for double digests, this c hart shows the optimal (supplied) NEBuffer and appr oximate activity in NEBuffer s and NEBuffer Ec oRI for eac h enzyme. If BSA is supplied with an enzyme, it is inc luded in all NEBuffer activity r eac tions table shows r ecommended reaction temperatur e, heat inactivation temper atur e, r ec ommended diluent buffer and whether the enzym qualified (i.e., cleaves substr ate in 5 m inutes under r ec ommended c onditions). N ote: The values listed in this table ar e appr oxima obtained using each enzyme's specific unit assay substr ate DNA. Please chec k out other technic al r efer enc e infor mation r elated to r estriction enzymes: Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffer s | Time-Saver Enzymes | High Fidelity (HF) Restriction Enz Click for the Chart Legend, Ic on Description A | B | C | D | E | F | H | I | K | L | M | N | P | R | S | T | X | Z | NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 1 NEBuffer 4 - NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 - NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 1 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 2 NEBuffer 4 NEBuffer 2 NEBuffer 1 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 2 NEBuffer 2 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 2 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 2 NEBuffer 4 NEBuffer 2 NEBuffer 3 NEBuffer 3 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4 Mix?- NEBuffer 2 NEBuffer 4 NEBuffer 3 NEBuffer 2 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 3 NEBuffer 4 NEBuffer DpnII NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 3 NEBuffer Ec Mix?- NEBuffer 4 NEBuffer 3 Mix?- NEBuffer 4 NEBuffer 2 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4 NEBuffer 4