猪卵母细胞体外成熟过程中cAMP和PKC对MAPK活性的影响
猪卵母细胞体外成熟过程中cAMP和PKC
对MAPK活性的影响
Proceedingofthe8MeetingoftheChineseReproductiveBiologists
EffectsofcAMPandPKConMAPKActivity
PorcineduringOocyteMaturationinvitro
LIMan-yu*’,FANHeng-yu,ToNGChao,CHENDa-yuan,
XIAGuo-liangandSUNQing-yuan
StateKeyLaboratoryofReproductiveBiology.InstituteofZoology,ChineseAcademy
ofSciences,BeOing100080,China
CollegeofBiologyandCollegeofAnimalMedicine.ChinaAgriculturalUniversity
Beijing100094,China
Besid~IAPKmanysignals,suchascAMPandPKC.areinvoIvedinoocytematuration.Byusing
SDS.PAGEandWesternblotting.wefoundthaIforskolinandIB慨
inhibitedtheactivationofMAPK
andtheoccurrenceofGVB0inporcineOOCytes.0nehundred.50and25uMOfforskolinorl0and5
mMofIBMXsignificantlyinhibitedtheactivationofMAPTwopMokadaica
cid.aspecific
inhib1torofproteinphosphainse.1and-2A.wereahieto?n僧rttheinhibi
咖.’reffectofr0rskolinor
IB
【ontheactivationofMAPKandmeiosisresumption.PMA,anactivatorofPKC,couldstronY
inhibittheactivationofKandtheocuurrenceofGVBDinporcineooevtas.162nMPMAat
completelyinhibitedtheactivationofMApK,andat1or1.62nMsnificantlyinhibitedthe
activationof
【AK.D.,aPhysiolocalPKcactivator,inhibitedtheactivationofMAPKandthe
occurredceofGVBD.1000Or500pMofdiinhibitedtheactivationofMAP1uMofcalphostinC,
aninhibitorofPKC,or30nMofstaurospoHne,anextensiveinhibitorofproteinkinases,overcamethe
effectOfPMAordiC,onoocytematuration.Biologicalinactivephorbolester.4ct-PDD.hadnoeffect
oneitherGVBDorlAPKactivation.Inconclusion.cAMPandPKCdown-regulatetheactivityof
MAPKduringpigoocvtematuration.
Keywords:poocyte,eJ~cl[P,PKCMAPK
猪卵母细胞体外成熟过程中cAMP乖PKC
对MAPK活性的影响
李满玉一”?范衡宇俦超陈太元夏图良一孙青原
+中国北京100080中国科学院动物研究所
计划
项目进度计划表范例计划下载计划下载计划下载课程教学计划下载
生育生殖生物学国家重点实验室
中国北京100094??中国农业犬学生物学院+++动物医学院
卵母细胞的成熟需要多种信号的调节,cAMPPKC和MAPK对卵母细胞的成熟具
有重要作用.通过体外培养猪的卵丘包裹的卵母细胞(COC)利用蛋白电泳和蛋白免疫
印迹技术,本文研究了猪卵母细胞体外成熟过程中cAMP和PKC对MAPK活性的影响
以及这三种信号通路对猪卵母细胞成熟的调节发现增加cAMP浓度的forskolin和1BMx
可抑制MAPK的活性和猪卵发生GvBD,100.50,25mforskolin或10,5rnMIBMx可
明显抑制MAPK的激活2/aM蛋白磷酸酶抑制剂OA可反转forskolin或IBMx对MAPK
激活的抑制PKC激活荆PMA可强烈抑制MAPK的激活和猪卵发生GVBD,162nM
PMA可完全抑制MAPK激活,l6.2,l62nMPMA可明显抑制MAPK的激活.无生理活
性的PMA类似物4a-pDD对MAPK激活和GVBD无任何影响生理性PKC激活剂diC
可抑制MAPK的激活和猪卵发生GVBD,1000,500pMPMA可明显抑制MAPK的激活.
1gMPKC抑制剂ca1FlhostinC或30nM广谱蛋白激酶抑制剂staurosporine可反转PMA或
diC对MAPK激活的抑制.以上结果揭示cAMP和PKC可负调节MAPK的激活,三者对
于猪卵成熟具有重要调节作用
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